| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTN2 |
| Uniprot No | P35609 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-894aa |
| 氨基酸序列 | MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERMEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSIMAIQNEVEKVIQSYNIRISSSNPYSTVTMDELRTKWDKVKQLVPIRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPAYSGPGSVPGALDYAAFSSALYGESDL |
| 预测分子量 | 103,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACTN2重组蛋白的3篇参考文献的简要信息：
1. **文献名称**：*Structural basis of the sarcomeric Z-disk assembly by α-actinin-2*
**作者**：Ribeiro E et al.
**摘要**：通过冷冻电镜解析了人源ACTN2重组蛋白的分子结构，揭示了其N端和C端结构域如何协同调控肌动蛋白交联功能，并探讨了心肌病相关突变对蛋白稳定性的影响。
2. **文献名称**：*Recombinant expression and functional characterization of α-actinin-2 in cardiomyocyte hypertrophy*
**作者**：Chen L, Wang H
**摘要**：成功在大肠杆菌中表达并纯化ACTN2重组蛋白，发现其在心肌细胞肥大模型中通过调节钙信号通路影响细胞收缩功能，为心肌病机制研究提供工具。
3. **文献名称**：*Interaction between α-actinin-2 and titin in the Z-disc region: A recombinant protein-based analysis*
**作者**：Kojima S et al.
**摘要**：利用重组ACTN2和肌联蛋白片段进行体外结合实验，证实二者通过特定结构域互作维持肌小节机械稳定性，并发现突变会显著降低结合亲和力。
注：以上文献信息为基于领域研究的概括性示例，实际文献需通过PubMed或Web of Science等数据库检索确认。
**Background of ACTN2 Recombinant Protein**
ACTN2. encoding alpha-actinin-2. is a cytoskeletal protein critical for maintaining structural integrity and functional dynamics in striated muscles. As a member of the spectrin superfamily, it forms antiparallel homodimers that crosslink actin filaments, stabilizing the sarcomeric Z-disc in cardiac and skeletal muscles. Beyond its mechanical role, ACTN2 interacts with signaling molecules, ion channels, and metabolic enzymes, influencing muscle contraction, force transmission, and mechanotransduction pathways.
Mutations in the ACTN2 gene are linked to cardiomyopathies (e.g., hypertrophic or dilated cardiomyopathy) and skeletal muscle disorders, highlighting its importance in muscle health. To study its functions and disease mechanisms, recombinant ACTN2 protein is engineered using expression systems like *E. coli* or mammalian cells. This involves cloning the human ACTN2 gene into vectors, followed by purification via affinity chromatography. Recombinant ACTN2 retains native structural domains, including actin-binding N-terminal CH domains and C-terminal calmodulin-like EF-hands, enabling *in vitro* studies on protein interactions, Z-disc assembly, or mutation effects.
Researchers utilize ACTN2 recombinant protein to explore pathological mechanisms, screen therapeutic compounds, or develop disease models. Its applications extend to structural biology (e.g., cryo-EM studies) and gene therapy research, where restoring wild-type ACTN2 expression is tested for rescuing muscle defects. Overall, ACTN2 recombinant protein serves as a vital tool for dissecting muscle biology and advancing treatments for actin-related myopathies.
×
