| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGN1 |
| Uniprot No | P05114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100aa |
| 氨基酸序列 | MPKRKVSSTEGAAKEEPKRRSARLSAKPPAKVEAKPKKAAAKDKSSDKKV QTKGKRGAKGKQAEVANQETKEDLPAENGETKTEESPASDEAGEKEAKSD |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGN1重组蛋白的3篇参考文献示例(内容基于典型研究方向，具体文献需进一步验证)：
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1. **文献名称**: *HMGN1 enhances chromatin accessibility and promotes transcriptional activation*
**作者**: Bustin M, et al.
**摘要**: 研究利用重组HMGN1蛋白，证明其通过降低核小体稳定性增加染色质可及性，促进转录因子结合，从而激活靶基因表达。
2. **文献名称**: *HMGN1 modulates NF-κB signaling and inflammatory responses*
**作者**: Agresti A, et al.
**摘要**: 通过体外实验发现，重组HMGN1蛋白可增强NF-κB与染色质结合能力，调控促炎因子表达，揭示其在炎症反应中的表观遗传调控机制。
3. **文献名称**: *Recombinant HMGN1 facilitates DNA repair via histone modification regulation*
**作者**: Postnikov Y, et al.
**摘要**: 研究表明，重组HMGN1通过影响组蛋白H3K36甲基化修饰，促进DNA损伤修复复合体募集，揭示其在基因组稳定性中的作用。
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**注**: 以上为示例性内容，实际文献需通过学术数据库检索确认。建议使用关键词“HMGN1 recombinant protein”在PubMed或Google Scholar中查找具体研究。
HMGN1 (High Mobility Group Nucleosome-Binding Protein 1) is a member of the HMGN protein family, which plays a critical role in modulating chromatin structure and regulating genomic functions. As a non-histone chromosomal protein, HMGN1 binds specifically to nucleosomes, the fundamental units of chromatin, and reduces the compaction of the chromatin fiber. This activity enhances DNA accessibility for transcription, replication, and repair processes. HMGN1 achieves this by competing with linker histone H1 for nucleosome binding sites, thereby destabilizing higher-order chromatin organization.
Recombinant HMGN1 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or other heterologous systems, followed by purification to ensure high quality and activity. Its recombinant form retains the native protein’s functional domains, including the nucleosome-binding domain and the chromatin-unfolding motif, enabling its use in *in vitro* studies. Researchers employ recombinant HMGN1 to investigate its role in epigenetic regulation, gene expression, and chromatin dynamics. It is also utilized to explore HMGN1’s involvement in cellular processes such as differentiation, development, and stress responses.
Dysregulation of HMGN1 has been linked to diseases, including cancer and inflammatory disorders, making its recombinant form valuable for mechanistic studies and drug discovery. By providing a controlled, pure protein source, recombinant HMGN1 facilitates biochemical assays, structural analyses, and functional genomics research, advancing our understanding of chromatin biology and its therapeutic implications.
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