| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTSR1 |
| Uniprot No | P30989 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MRLNSSAPGTPGTPAADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVYLALFVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVNTFMSFIFPMVVISVLNTIIANKLTVMVRQAAEQGQVCTVGGEHSTFSMAIEPGRVQALRHGVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYFYMVTNALFYVSSTINPILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSSNHTLSSNATRETLY |
| 预测分子量 | 47.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及NTSR1重组蛋白研究的代表性文献摘要概括(基于公开论文数据模拟，具体文献请以实际数据库检索为准)：
1. **《Cryo-EM structure of neurotensin receptor 1 reveals molecular mechanism of neuropeptide binding》**
- 作者：Egloff, P. et al.
- 摘要：通过冷冻电镜解析了NTSR1重组蛋白与神经降压素结合的复合体结构，揭示了受体激活过程中肽类配体的结合口袋构象变化及G蛋白偶联机制。
2. **《Expression and purification of functional human neurotensin receptor 1 in Escherichia coli》**
- 作者：Zhang, Y. et al.
- 摘要：开发了一种在大肠杆菌中高效表达NTSR1重组蛋白的方法，通过膜蛋白复性技术获得具有配体结合活性的受体，为大规模制备提供新策略。
3. **《Structural insights into the activation of neurotensin receptor 1 by small molecule agonists》**
- 作者：Shiroishi, M. et al.
- 摘要：利用昆虫细胞表达系统获得NTSR1重组蛋白，结合X射线晶体学阐明了小分子激动剂诱导的受体跨膜结构域构象变化，指导了靶向该受体的药物设计。
注：以上文献标题及内容为基于领域研究的模拟概括，实际文献请通过PubMed/SciHub等平台检索关键词"NTSR1 recombinant protein"或结合具体研究方向筛选。
**Background of NTSR1 Recombinant Protein**
The neurotensin receptor type 1 (NTSR1) is a G protein-coupled receptor (GPCR) that binds neurotensin (NT), a 13-amino-acid neuropeptide involved in diverse physiological processes, including neurotransmission, pain modulation, and gastrointestinal functions. NTSR1 is primarily expressed in the central nervous system (CNS) and peripheral tissues, such as the gut, where it mediates cellular responses through Gq/11-dependent signaling pathways. Activation of NTSR1 triggers phospholipase C (PLC) activation, leading to increased intracellular calcium levels and protein kinase C (PKC) activity. Dysregulation of NTSR1 signaling has been implicated in neurological disorders (e.g., schizophrenia, Parkinson’s disease), cancer progression, and metabolic syndromes, highlighting its therapeutic potential.
Recombinant NTSR1 protein is engineered for structural and functional studies to elucidate receptor-ligand interactions, signaling mechanisms, and drug discovery. Produced in heterologous expression systems (e.g., insect or mammalian cells), the recombinant protein retains native receptor conformation, enabling biochemical assays, ligand binding studies, and high-resolution structural analyses (e.g., cryo-EM, X-ray crystallography). These studies have revealed critical insights into NT binding modes and receptor activation, aiding the design of NTSR1-targeted therapeutics.
Recent advancements in GPCR research have positioned NTSR1 as a model for studying biased signaling and allosteric modulation. Challenges in producing stable, functional NTSR1 recombinant protein—due to its hydrophobic transmembrane domains—have driven innovations in membrane protein expression and purification. Current efforts focus on developing NTSR1-selective agonists/antagonists for treating pain, addiction, and cancers, leveraging recombinant protein tools to accelerate translational research.
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