| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMNB1 |
| Uniprot No | P20700 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-583aa |
| 氨基酸序列 | ATATPVPPR MGSRAGGPTT PLSPTRLSRL QEKEELRELN DRLAVYIDKV RSLETENSAL QLQVTEREEV RGRELTGLKA LYETELADAR RALDDTARER AKLQIELGKC KAEHDQLLLN YAKKESDLNG AQIKLREYEA ALNSKDAALA TALGDKKSLE GDLEDLKDQI AQLEASLAAA KKQLADETLL KVDLENRCQS LTEDLEFRKS MYEEEINETR RKHETRLVEV DSGRQIEYEY KLAQALHEMR EQHDAQVRLY KEELEQTYHA KLENARLSSE MNTSTVNSAR EELMESRMRI ESLSSQLSNL QKESRACLER IQELEDLLAK EKDNSRRMLT DKEREMAEIR DQMQQQLNDY EQLLDVKLAL DMEISAYRKL LEGEEERLKL SPSPSSRVTV SRASSSRSVR TTRGKRKRVD VEESEASSSV SISHSASATG NVCIEEIDVD GKFIRLKNTS EQDQPMGGWE MIRKIGDTSV SYKYTSRYVL KAGQTVTIWA ANAGVTASPP TDLIWKNQNS WGTGEDVKVI LKNSQGEEVA QRSTVFKTTI PEEEEEEEEA AGVVVEEELF HQQGTPRASN RSC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LMNB1重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**：*"Recombinant human lamin B1 for structural and functional studies"*
**作者**：Smith et al.
**摘要**：该研究报道了利用昆虫细胞表达系统高效表达和纯化重组人源LMNB1蛋白的方法，并利用冷冻电镜技术解析其与染色质相互作用的分子机制，揭示了其在维持核膜稳定性中的关键作用。
2. **标题**：*"Lamin B1 reorganization during cellular senescence is regulated by its phosphorylation status"*
**作者**：Shimi et al.
**摘要**：通过表达重组LMNB1蛋白及模拟磷酸化突变体，研究证明LMNB1的磷酸化修饰影响其在细胞衰老过程中的定位变化，进而调控核纤层结构解体和衰老相关异染色质聚集。
3. **标题**：*"In vitro reconstitution of nuclear lamina assembly using recombinant lamin B1"*
**作者**：Dechat et al.
**摘要**：利用重组LMNB1蛋白在体外重建核纤层组装过程，发现其与核膜蛋白(如emerin)的互作是维持核膜机械稳定性的必要条件，为核纤层相关疾病机制提供了模型支持。
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**备注**：上述文献为示例，实际引用时需根据具体研究内容核实标题、作者及摘要准确性。建议通过PubMed或Web of Science以关键词“recombinant LMNB1”或“lamin B1 recombinant protein”检索最新研究。
**Background of LMNБ1 Recombinant Protein**
Lamin B1 (LMNБ1) is a key structural protein of the nuclear lamina, a meshwork underlying the inner nuclear membrane that provides mechanical stability to the nucleus, regulates chromatin organization, and influences gene expression. Encoded by the *LMNB1* gene in humans, it belongs to the B-type lamin family, which also includes lamin B2. Unlike A-type lamins, B-type lamins are expressed throughout development and are essential for cell viability. LMNБ1 plays critical roles in maintaining nuclear shape, mitotic spindle assembly, and DNA replication. It also participates in cellular processes such as senescence, apoptosis, and response to mechanical stress.
Recombinant LMNБ1 protein is produced using expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical properties and interactions in vitro. Its recombinant form is often tagged (e.g., His-tag) for purification and detection. Studies using recombinant LMNБ1 have shed light on its role in nuclear envelope reassembly during mitosis, interactions with chromatin modifiers, and involvement in age-related nuclear abnormalities. Dysregulation of LMNБ1 is linked to diseases, including premature aging disorders, neurodegenerative conditions, and cancers, where altered lamin expression disrupts nuclear integrity and cellular function.
Research applications of recombinant LMNБ1 include investigating laminopathies, modeling nuclear structural defects, and screening therapeutic compounds. It is also used to explore LMNБ1's role in viral infection mechanisms, as some pathogens exploit the nuclear lamina for replication. Recent advances in CRISPR and gene-editing technologies further emphasize the utility of recombinant LMNБ1 in mechanistic studies and drug development. Quality-controlled batches ensure consistency in experiments, supporting both basic research and translational efforts targeting lamin-associated diseases.
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