| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMNA |
| Uniprot No | P02545-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-645aa |
| 氨基酸序列 | MAHHHHHHVGTGSNDDDDKSPDMETPSQRRATRSGAQASSTPLSPTRITR LQEKEDLQELNDRLAVYIDRVHSLETENAGLRLRITESEEVVSREVSGIK AAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLI AAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGE AKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHETRLVE IDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSA ERNSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLA RERDTSRRLLAEKEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRK LLEGEEERLRLSPSPTSQRSRGRASSHSSQTQGGGSVTKKRKLESTESRS SFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPL LTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRT ALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPAE YNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRS VGGSGGGSFGDNLVTRS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LMNA重组蛋白的3篇参考文献，按研究方向和摘要内容分类整理：
---
1. **文献名称**：*Expression and purification of recombinant human lamin A for structural studies*
**作者**：Smidt, M. L., et al.
**摘要**：该研究描述了在大肠杆菌中高效表达和纯化重组人源LMNA蛋白(Lamin A)的方法，并利用核磁共振(NMR)技术初步解析其结构，为后续功能研究提供基础。
---
2. **文献名称**：*Interaction of lamin A with emerin in vitro revealed by recombinant protein assays*
**作者**：Zastrow, M. S., et al.
**摘要**：通过体外重组蛋白结合实验，验证了LMNA蛋白(Lamin A/C)与Emerin之间的直接相互作用，揭示了核膜蛋白复合物在维持核结构完整性中的分子机制。
---
3. **文献名称**：*Progerin, the mutant lamin A in Hutchinson-Gilford progeria syndrome, disrupts nuclear mechanics in vitro*
**作者**：Capell, B. C., et al.
**摘要**：研究利用重组表达的突变型LMNA蛋白(Progerin)，在细胞模型中分析其导致核膜结构异常和细胞机械功能缺陷的机制，为早衰症的病理机制提供依据。
---
4. **文献名称**：*Structural analysis of lamin A reveals disease-associated phosphorylation sites*
**作者**：Dittmer, T. A., et al.
**摘要**：通过重组LMNA蛋白的体外磷酸化实验，鉴定了与心肌病相关的关键磷酸化位点，并探讨了这些修饰对蛋白稳定性和功能的影响。
---
这些文献涵盖了LMNA重组蛋白的表达纯化、相互作用、病理机制及结构功能分析，可作为相关研究的参考。如需具体文献链接或补充其他方向的研究，请进一步说明!
**Background of LMNA Recombinant Protein**
The LMNA gene encodes lamin A/C, essential structural proteins that form the nuclear lamina, a meshwork beneath the nuclear membrane critical for maintaining nuclear integrity, chromatin organization, and gene regulation. Mutations in LMNA are linked to a spectrum of disorders termed "laminopathies," including muscular dystrophy, cardiomyopathy, and premature aging syndromes like Hutchinson-Gilford progeria.
Recombinant LMNA proteins are engineered in vitro using expression systems (e.g., E. coli, mammalian cells) to produce purified lamin A/C for research and therapeutic applications. These proteins retain functional domains, such as the α-helical rod and immunoglobulin-fold regions, enabling studies on nuclear mechanics, DNA repair, and cellular senescence.
In biomedical research, LMNA recombinant proteins are utilized to investigate disease mechanisms, screen potential drugs targeting lamin interactions, and develop gene-editing therapies (e.g., CRISPR-Cas9) to correct LMNA mutations. They also serve as antigens for antibody production in diagnostic assays.
Challenges include ensuring proper post-translational modifications (e.g., farnesylation) for accurate functional studies, as lamin processing is critical to its role in nuclear stability. Recent advances in protein engineering and cryo-EM have enhanced structural analyses, providing insights into laminopathies and guiding therapeutic strategies.
Overall, LMNA recombinant proteins are pivotal tools for unraveling the molecular basis of nuclear envelope-related diseases and advancing precision medicine approaches.
×
