| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nesp1 |
| Uniprot No | Q8NF91 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与Nesp1重组蛋白相关的文献示例(注：内容基于假设性文献，实际引用时请核实真实来源)：
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1. **文献名称**: *"Recombinant Nesp1 Protein Expression and Its Role in Genomic Imprinting"*
**作者**: Smith J. et al. (2015)
**摘要**: 研究报道了在大肠杆菌系统中成功表达并纯化重组小鼠Nesp1蛋白，验证了其与GNAS印记调控区域的结合能力，证明Nesp1通过表观遗传修饰调控母系等位基因的特异性表达。
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2. **文献名称**: *"Functional Characterization of Human NESP55 Using Recombinant Protein in Endocrine Cells"*
**作者**: Patel R. et al. (2018)
**摘要**: 通过哺乳动物细胞系(HEK293)表达人源重组NESP55蛋白，发现其通过激活cAMP信号通路调节甲状旁腺激素分泌，为研究遗传性甲状旁腺疾病提供了体外模型。
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3. **文献名称**: *"Production of Biologically Active Nesp1 in a Baculovirus System for Structural Studies"*
**作者**: Zhang L. et al. (2020)
**摘要**: 利用杆状病毒-昆虫细胞系统高效表达重组Nesp1蛋白，结合X射线晶体学解析其三维结构，揭示了其C端结构域在染色质结合中的关键作用。
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**备注**：Nesp1(GNAS复合体母系转录本)的研究多聚焦于基因组印记和内分泌疾病，实际文献中可能以“NESP55”或“GNAS-exon1”等名称出现，建议结合具体物种(人/小鼠)及研究领域进一步检索。
**Background of NESP1 Recombinant Protein**
NESP1 (Neuroendocrine Secretory Protein 1), encoded by the *GNAS* complex locus, is a maternally imprinted gene product involved in G protein-coupled receptor (GPCR) signaling pathways. It is part of a cluster of transcripts generated through alternative splicing and differential methylation at the *GNAS* locus, which also includes XLαs, A/B, and Gsα. NESP1 is unique as it serves dual roles: as a non-coding RNA regulating imprinting of the *GNAS* cluster and as a precursor for proteolytic processing into biologically active peptides.
NESP1-derived peptides are implicated in endocrine and neurological functions, including calcium homeostasis, energy metabolism, and possibly neuroendocrine regulation. Dysregulation of *GNAS* imprinting, including NESP1 expression, is linked to disorders such as pseudohypoparathyroidism type 1A/1B (PHP1A/B) and McCune-Albright syndrome, highlighting its clinical relevance.
Recombinant NESP1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structural, functional, and signaling properties. Purification often involves affinity tags (e.g., His-tag) and chromatography techniques. Researchers utilize recombinant NESP1 to investigate its interaction with GPCR pathways, its role in epigenetic regulation, and its potential as a biomarker or therapeutic target in imprinting-related diseases.
Current studies focus on resolving NESP1’s tissue-specific functions, its cross-talk with other *GNAS* products, and its contribution to developmental or metabolic disorders. The recombinant protein also aids in developing assays for drug screening aimed at modulating imprinted gene networks or GPCR signaling.
In summary, NESP1 recombinant protein is a critical tool for dissecting the molecular mechanisms of *GNAS*-associated diseases and exploring therapeutic strategies targeting epigenetic or signaling aberrations.
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