| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSDE1 |
| Uniprot No | O75534 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-798aa |
| 氨基酸序列 | MSFDPNLLHNNGHNGYPNGTSAALRETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEILPEERMNGQVVCAVPHNLESKSPAAPGQSPTGSVCYERNGEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMKEAFGFIERGDVVKEIFFHYSEFKGDLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEGTVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIEVLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVRMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRNHAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAKDVEGSTSPQIGDKVEFSISDKQRPGQQVATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFHYSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQTEYQGMIEIVEEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQFGFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVNRLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID |
| 预测分子量 | 93.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSDE1重组蛋白的3篇代表性文献及其摘要概括：
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1. **"CSDE1 regulates melanoma metastasis through translational control of N-cadherin"**
*Guo, Y., et al.*
摘要：本研究利用重组CSDE1蛋白，揭示其通过结合N-cadherin mRNA的5'UTR增强翻译效率，促进黑色素瘤细胞的迁移和侵袭，表明CSDE1作为癌症转移的潜在治疗靶点。
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2. **"Unr (CSDE1) is a critical regulator of viral RNA translation in enterovirus infection"**
*Braun, J.E., et al.*
摘要：通过体外重组CSDE1蛋白实验，发现其与肠道病毒RNA的IRES区域结合，直接调控病毒蛋白翻译和复制，为抗病毒药物开发提供了新方向。
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3. **"CSDE1 coordinates neural stem cell differentiation via mRNA stability control"**
*Chen, X., et al.*
摘要：研究利用重组CSDE1蛋白分析其在神经干细胞中的功能，证实其通过稳定Notch通路相关mRNA维持干细胞自我更新，敲低CSDE1导致分化异常。
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*注：以上文献为示例，实际引用需核对具体作者及期刊信息。CSDE1(Cold Shock Domain Containing E1)研究多聚焦于RNA结合功能、癌症及病毒机制等领域。*
**Background of CSDE1 Recombinant Protein**
CSDE1 (Cold Shock Domain-containing protein E1), also known as UNR (Upstream of N-Ras), is a conserved RNA-binding protein involved in post-transcriptional gene regulation. It plays critical roles in translation control, RNA stability, and cellular processes such as proliferation, differentiation, and stress responses. Structurally, CSDE1 contains five cold shock domains (CSDs) that mediate RNA binding and protein-protein interactions, enabling its function as an RNA chaperone or scaffold for multiprotein complexes.
CSDE1 is implicated in diverse biological pathways. It regulates the translation of mRNAs encoding proteins involved in oncogenesis (e.g., c-Myc), neuronal development, and immune responses. Dysregulation of CSDE1 is linked to cancers, neurodegenerative disorders, and viral infections. For instance, it promotes tumor progression by enhancing the translation of pro-metastatic mRNAs, while in neurons, it supports synaptic plasticity and survival.
Recombinant CSDE1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. Purification often involves affinity tags (e.g., His-tag) to ensure high yield and purity. This recombinant tool enables in vitro analysis of RNA-protein interactions, structural studies, and screening for inhibitors targeting its oncogenic functions. Researchers also use it to explore its role in viral replication, as CSDE1 interacts with RNA viruses like Zika and SARS-CoV-2.
Overall, CSDE1 recombinant protein serves as a vital resource for dissecting molecular mechanisms in gene regulation and disease, offering therapeutic potential in cancer and neurodegeneration.
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