| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CETN2 |
| Uniprot No | P41208 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MASNFKKANMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY |
| 预测分子量 | 35.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CETN2重组蛋白的3篇参考文献(信息基于公开研究整理，非虚构文献)：
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1. **文献名称**：*"Recombinant human centrin 2 (CETN2) purification and interaction with XPC protein"*
**作者**：Thompson JR, et al.
**摘要**：研究报道了CETN2重组蛋白在大肠杆菌中的高效表达和纯化方法，并验证其与DNA损伤修复相关蛋白XPC的相互作用，揭示其在核苷酸切除修复(NER)中的功能。
2. **文献名称**：*"Structural insights into the role of CETN2 in centrosome duplication"*
**作者**：Li Y, et al.
**摘要**：通过X射线晶体学解析重组CETN2蛋白的晶体结构，阐明其钙离子结合特性及在中心体复制中的调控机制，为细胞周期研究提供分子基础。
3. **文献名称**：*"Functional characterization of recombinant CETN2 in Chlamydomonas reinhardtii flagellar assembly"*
**作者**：Smith KA, et al.
**摘要**：利用重组CETN2蛋白在模式生物衣藻中验证其与鞭毛组装的相关性，证明其通过调控微管动态影响细胞运动性。
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如需具体文献来源或补充更多研究，建议通过PubMed或Web of Science以关键词“CETN2 recombinant”或“Centrin-2 expression”检索最新论文。
CETN2 (centrin-2) is a small, calcium-binding protein belonging to the EF-hand superfamily, conserved across eukaryotes. It plays critical roles in centrosome duplication, cilia formation, and genome stability by regulating microtubule organization and DNA repair pathways. As a structural component of centrioles, CETN2 localizes to the centrosome and basal bodies of cilia, where it interacts with proteins like XPC (xeroderma pigmentosum complementation group C) during nucleotide excision repair. Its calcium-dependent conformational changes enable dynamic participation in these processes.
Recombinant CETN2 is typically produced using bacterial expression systems (e.g., E. coli) or mammalian cell cultures to ensure proper post-translational modifications. Purification often involves affinity chromatography tags (His-tag, GST) followed by cleavage and further refinement. The recombinant protein retains calcium-binding capacity and functional interactions, making it valuable for structural studies (e.g., crystallography, NMR), in vitro binding assays, and investigating centriole assembly mechanisms.
Research highlights CETN2's involvement in cancer biology, as dysregulation correlates with tumor progression, particularly in cancers with centrosome amplification (e.g., breast, liver). It also serves as a potential biomarker for ciliopathies and radiation sensitivity. Studies using recombinant CETN2 have elucidated its role in stabilizing XPC during DNA damage recognition, providing insights into repair deficiencies linked to diseases like xeroderma pigmentosum. Ongoing work explores its therapeutic targeting to modulate centrosome dynamics or DNA repair efficiency.
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