| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FATP1 |
| Uniprot No | Q6PCB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-646aa |
| 氨基酸序列 | MRAPGAGAASVVSLALLWLLGLPWTWSAAAALGVYVGSGGWRFLRIVCKT ARRDLFGLSVLIRVRLELRRHQRAGHTIPRIFQAVVQRQPERLALVDAGT GECWTFAQLDAYSNAVANLFRQLGFAPGDVVAIFLEGRPEFVGLWLGLAK AGMEAALLNVNLRREPLAFCLGTSGAKALIFGGEMVAAVAEVSGHLGKSL IKFCSGDLGPEGILPDTHLLDPLLKEASTAPLAQIPSKGMDDRLFYIYTS GTTGLPKAAIVVHSRYYRMAAFGHHAYRMQAADVLYDCLPLYHSAGNIIG VGQCLIYGLTVVLRKKFSASRFWDDCIKYNCTVVQYIGEICRYLLKQPVR EAERRHRVRLAVGNGLRPAIWEEFTERFGVRQIGEFYGATECNCSIANMD GKVGSCGFNSRILPHVYPIRLVKVNEDTMELLRDAQGLCIPCQAGEPGLL VGQINQQDPLRRFDGYVSESATSKKIAHSVFSKGDSAYLSGDVLVMDELG YMYFRDRSGDTFRWRGENVSTTEVEGVLSRLLGQTDVAVYGVAVPGVEGK AGMAAVADPHSLLDPNAIYQELQKVLAPYARPIFLRLLPQVDTTGTFKIQ KTRLQREGFDPRQTSDRLFFLDLKQGHYLPLNEAVYTRICSGAFAL |
| 预测分子量 | 98 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FATP1重组蛋白的文献摘要概述：
1. **文献名称**: "Functional characterization of recombinant murine fatty acid transport protein 1 in vitro"
**作者**: Stahl A et al.
**摘要**: 研究利用昆虫细胞表达系统成功表达并纯化重组小鼠FATP1蛋白，证实其具有长链脂肪酸(LCFA)转运活性，并发现其酶活性依赖ATP水解，揭示了FATP1在脂肪酸代谢中的双重功能。
2. **文献名称**: "Purification and biochemical analysis of human FATP1: insights into its acyl-CoA synthetase activity"
**作者**: Hall AM et al.
**摘要**: 通过哺乳动物表达系统获得人源重组FATP1.发现其不仅促进脂肪酸跨膜转运，还具有酰基辅酶A合成酶活性，表明FATP1通过酶促反应参与脂肪酸的细胞内代谢调控。
3. **文献名称**: "FATP1 overexpression enhances cellular lipid uptake via recombinant protein-mediated membrane localization"
**作者**: Wu Q et al.
**摘要**: 研究通过HEK293细胞表达重组FATP1.证明其过表达显著增加细胞对荧光标记脂肪酸的吸收效率，并通过免疫荧光定位发现FATP1主要定位于细胞膜和脂滴表面，提示其空间分布与功能密切相关。
(注：以上为模拟文献，实际检索需通过PubMed/Google Scholar等平台验证)
**Background of FATP1 Recombinant Protein**
Fatty Acid Transport Protein 1 (FATP1), encoded by the *SLC27A1* gene, is a transmembrane protein critical for cellular uptake and metabolism of long-chain fatty acids (LCFAs). It belongs to the solute carrier family 27 (SLC27), which comprises six isoforms with distinct tissue distributions. FATP1 is predominantly expressed in metabolically active tissues, including adipose tissue, skeletal muscle, heart, and liver, where it facilitates fatty acid transport across the plasma membrane and their subsequent activation into acyl-CoA esters for energy production or storage.
Structurally, FATP1 contains an N-terminal AMP-binding domain and a conserved fatty acid-binding motif, enabling both transport and enzymatic acyl-CoA synthetase activity. This dual function links fatty acid internalization to metabolic pathways, influencing lipid homeostasis. Dysregulation of FATP1 is implicated in metabolic disorders such as insulin resistance, obesity, and type 2 diabetes. Studies in *FATP1-knockout* mice reveal improved insulin sensitivity and reduced lipid accumulation, highlighting its role in lipid-induced metabolic stress.
Recombinant FATP1 protein is produced using expression systems like *E. coli* or mammalian cells, often tagged with affinity markers (e.g., His-tag) for purification. It serves as a tool to study fatty acid transport mechanisms, screen lipid-lowering therapeutics, or investigate molecular interactions in cellular models. Its applications extend to drug development targeting metabolic diseases, emphasizing FATP1's potential as a therapeutic target. Research continues to explore its regulatory pathways and tissue-specific functions, aiming to address obesity-related pathologies.
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