| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADIPOR2 |
| Uniprot No | Q86V24 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386aa |
| 氨基酸序列 | MNEPTENRLGCSRTPEPDIRLRKGHQLDGTRRGDNDSHQGDLEPILEASVLSSHHKKSSEEHEYSDEAPQEDEGFMGMSPLLQAHHAMEKMEEFVCKVWEGRWRVIPHDVLPDWLKDNDFLLHGHRPPMPSFRACFKSIFRIHTETGNIWTHLLGCVFFLCLGIFYMFRPNISFVAPLQEKVVFGLFFLGAILCLSFSWLFHTVYCHSEGVSRLFSKLDYSGIALLIMGSFVPWLYYSFYCNPQPCFIYLIVICVLGIAAIIVSQWDMFATPQYRGVRAGVFLGLGLSGIIPTLHYVISEGFLKAATIGQIGWLMLMASLYITGAALYAARIPERFFPGKCDIWFHSHQLFHIFVVAGAFVHFHGVSNLQEFRFMIGGGCSEEDAL |
| 预测分子量 | 43,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADIPOR2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant expression and functional characterization of human adiponectin receptor 2 (ADIPOR2) in insulin resistance"*
**作者**: Yamauchi T, et al.
**摘要**: 研究通过哺乳动物细胞系统重组表达了人源ADIPOR2蛋白，验证其与脂联素结合的能力，并证明其在调节胰岛素敏感性和脂肪酸氧化中的关键作用，为代谢疾病治疗提供靶点依据。
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2. **文献名称**: *"Structural insights into ADIPOR2 signaling mechanism through cryo-EM analysis"*
**作者**: Tanabe H, et al.
**摘要**: 利用冷冻电镜技术解析ADIPOR2重组蛋白的三维结构，揭示其与脂联素及下游效应分子结合的构象变化，阐明其在AMPK/PPARα信号通路中的分子机制。
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3. **文献名称**: *"ADIPOR2 recombinant protein attenuates hepatic steatosis via autophagy activation in high-fat diet-induced mice"*
**作者**: Zhou L, et al.
**摘要**: 通过大肠杆菌表达系统制备重组ADIPOR2蛋白，验证其在动物模型中通过激活自噬通路减轻肝脏脂质堆积的效果，提示其在非酒精性脂肪肝治疗中的潜在价值。
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**注**：以上文献信息为示例性概括，实际引用需根据具体研究内容检索真实文献(如PubMed或Web of Science数据库)。
ADIPOR2 (Adiponectin Receptor 2) is a membrane-bound protein that belongs to the adiponectin receptor family, primarily involved in regulating metabolic processes such as glucose homeostasis and fatty acid oxidation. It binds to adiponectin, an adipocyte-secreted hormone with insulin-sensitizing, anti-inflammatory, and anti-diabetic properties. ADIPOR2 is widely expressed in metabolically active tissues, including the liver, skeletal muscle, and adipose tissue, where it activates downstream signaling pathways like AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor alpha (PPARα). These pathways enhance energy metabolism, improve insulin sensitivity, and reduce lipid accumulation.
Recombinant ADIPOR2 protein is engineered in vitro using expression systems such as Escherichia coli, yeast, or mammalian cells to produce purified, functional forms of the receptor for research and therapeutic applications. It typically retains key structural features, including seven transmembrane domains, though it differs from G protein-coupled receptors (GPCRs) in topology and signaling mechanisms. Researchers utilize recombinant ADIPOR2 to study ligand-receptor interactions, signal transduction mechanisms, and its role in metabolic disorders like obesity, type 2 diabetes, and non-alcoholic fatty liver disease (NAFLD). It also serves as a tool for drug screening to identify agonists or modulators targeting metabolic syndromes. Additionally, structural studies of recombinant ADIPOR2 have provided insights into its unique mechanism of adiponectin binding and downstream effector recruitment. Its therapeutic potential lies in developing strategies to mimic adiponectin signaling, offering avenues for treating insulin resistance and related comorbidities.
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