| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LSR |
| Uniprot No | Q86X29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-259aa |
| 氨基酸序列 | MQQDGLGVGTRNGSGKGRSVHPSWPWCAPRPLRYFGRDARARRAQTAAMALLAGGLSRGLGSHPAAAGRDAVVFVWLLLSTWCTAPARAIQVTVSNPYHVVILFQPVTLPCTYQMTSTPTQPIVIWKYKSFCRDRIADAFSPASVDNQLNAQLAAGNPGYNPYVECQDSVRTVRVVATKQGNAVTLGDYYQGRRITITGNADLTFDQTAWGDSGVYYCSVVSAQDLQGNNEAYAELIVLGRTSGVAELLPGFQAGPIED |
| 预测分子量 | 31.9kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LSR(Lipolysis-Stimulated Lipoprotein Receptor)重组蛋白的参考文献示例，涵盖其功能研究、表达纯化及结构解析：
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1. **文献名称**: *Lipolysis-stimulated lipoprotein receptor: a novel membrane protein of hepatocytes that mediates the uptake of remnant lipoproteins*
**作者**: Yen FT, et al.
**摘要**: 该研究首次克隆并鉴定了LSR在肝细胞中的表达，揭示其通过介导残余脂蛋白的摄取参与脂代谢调控，为后续重组蛋白的功能研究提供了基础。
2. **文献名称**: *Expression and functional characterization of recombinant human LSR in mammalian cell systems*
**作者**: Smith JL, et al.
**摘要**: 报道了在HEK293细胞中重组表达人源LSR蛋白的优化方法，通过亲和层析纯化获得功能性蛋白，并验证其与脂蛋白结合的能力。
3. **文献名称**: *Structural insights into ligand recognition by the extracellular domain of LSR*
**作者**: Zhang Q, et al.
**摘要**: 利用重组表达的LSR胞外域解析其晶体结构，揭示了该受体与脂质配体(如极低密度脂蛋白)结合的分子机制。
4. **文献名称**: *Recombinant LSR protein enhances hepatic clearance of triglyceride-rich lipoproteins in murine models*
**作者**: Wang H, et al.
**摘要**: 在小鼠模型中验证重组LSR蛋白的治疗潜力，证明其通过促进肝脏摄取甘油三酯富集颗粒改善高脂血症。
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**说明**：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以关键词“LSR recombinant protein”“lipolysis-stimulated lipoprotein receptor”等检索最新文献。若需具体文献，可进一步提供研究方向或应用场景。
**Background of LSR Recombinant Protein**
The Lipolysis-Stimulated Lipoprotein Receptor (LSR), initially identified for its role in lipid metabolism, is a transmembrane protein involved in cellular uptake of triglyceride-rich lipoproteins during the postprandial phase. Structurally, LSR belongs to the immunoglobulin superfamily and functions as a component of tricellular tight junctions in epithelial and endothelial cells, contributing to barrier integrity and cell polarity. Its dual functionality—linking lipid homeostasis and cell-cell adhesion—has spurred interest in metabolic diseases, cancer, and tissue development research.
Recombinant LSR proteins are engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional LSR for *in vitro* and *in vivo* studies. These proteins retain critical domains, such as the extracellular immunoglobulin-like folds and intracellular signaling motifs, enabling researchers to investigate ligand-receptor interactions, structural dynamics, and downstream pathways.
LSR's involvement in diseases like obesity, diabetes, and metastatic cancers underscores its therapeutic potential. For instance, dysregulated LSR expression correlates with altered lipid absorption and cancer cell dissemination. Recombinant LSR facilitates drug screening, antibody development, and mechanistic studies targeting metabolic syndromes or tumor microenvironments.
Recent advances in cryo-EM and crystallography have utilized recombinant LSR to resolve its oligomeric structure and binding interfaces, enhancing understanding of its role in cellular signaling and barrier function. As a research tool, recombinant LSR bridges gaps between molecular biology and translational medicine, offering insights into metabolic regulation and tissue homeostasis.
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