| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCN6 |
| Uniprot No | P62502 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-163aa |
| 氨基酸序列 | VWLGRLDPEQLLGPWYVLAVASREKGFAMEKDMKNVVGVVVTLTPENNLRTLSSQHGLGGCDQSVMDLIKRNSGWVFENPSIGVLELWVLATNFRDYAIIFTQLEFGDEPFNTVELYSLTETASQEAMGLFTKWSRSLGFLSQ |
| 预测分子量 | 23.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LCN6重组蛋白的3篇文献示例(注：以下内容基于领域内常见研究方向模拟整理，实际文献需通过学术数据库查询确认)：
1. **《Lipocalin 6 regulates sperm function via binding to fatty acids》**
*作者：Smith A, et al.*
摘要：该研究通过重组表达人源LCN6蛋白，发现其能够特异性结合不饱和脂肪酸，并在体外实验中证明LCN6重组蛋白可增强精子运动能力，提示其在男性生殖系统中的潜在作用。
2. **《Expression and purification of recombinant LCN6 for structural analysis》**
*作者：Zhang Y, et al.*
摘要：报道了利用大肠杆菌系统高效表达LCN6重组蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并利用圆二色谱分析其二级结构，为后续功能研究提供基础。
3. **《LCN6 overexpression in breast cancer cells promotes invasion through MMP-9 activation》**
*作者：Wang X, et al.*
摘要：研究构建了LCN6重组真核表达载体，转染乳腺癌细胞后发现其通过上调MMP-9促进肿瘤细胞侵袭，提示LCN6可能成为癌症治疗的潜在靶点。
(提示：若需具体文献，建议通过PubMed/Google Scholar检索关键词“LCN6 recombinant”或“lipocalin 6 protein expression”获取最新研究。)
**Background of LCN6 Recombinant Protein**
Lipocalin 6 (LCN6) is a member of the lipocalin protein family, a group of small, secreted proteins characterized by their ability to bind and transport hydrophobic molecules, such as lipids, steroids, and retinoids. The lipocalin family plays diverse roles in cellular processes, including immune response, metabolism, and signal transduction. LCN6. specifically, is encoded by the *LCN6* gene and is primarily expressed in reproductive tissues, such as the epididymis, suggesting a potential role in sperm maturation or fertility.
Structurally, LCN6 shares the conserved lipocalin fold—a β-barrel structure that forms a ligand-binding pocket. This configuration enables interactions with small molecules, though its precise physiological ligands remain under investigation. Recombinant LCN6 protein is produced using biotechnological methods, such as expression in bacterial or mammalian systems, followed by purification to ensure high specificity and activity. This engineered protein retains the functional properties of native LCN6. making it valuable for in vitro and in vivo studies.
Research on LCN6 has gained interest due to its tissue-specific expression and potential clinical relevance. Studies suggest its involvement in male reproductive health, with altered expression linked to infertility. Additionally, lipocalins are explored for their diagnostic and therapeutic potential, as their binding capabilities can be harnessed for drug delivery or biomarker development. However, the exact mechanisms of LCN6 in cellular pathways and disease contexts require further elucidation.
Overall, recombinant LCN6 serves as a critical tool for deciphering the protein’s biological functions and its applications in reproductive medicine and beyond. Ongoing studies aim to clarify its ligand interactions, signaling roles, and utility in addressing fertility challenges or related disorders.
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