| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCN12 |
| Uniprot No | Q6JVE5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-192aa |
| 氨基酸序列 | Q TPTPLPLPPP MQSFQGNQFQ GEWFVLGLAG NSFRPEHRAL LNAFTATFEL SDDGRFEVWN AMTRGQHCDT WSYVLIPAAQ PGQFTVDHGV EPGADREETR VVDSDYTQFA LMLSRRHTSR LAVLRISLLG RSWLLPPGTL DQFICLGRAQ GLSDDNIVFP DVTGWSPQAS VC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LCN12重组蛋白的假设性参考文献示例(基于常见研究方向的合理推测，实际文献需进一步验证)：
1. **《重组人LCN12蛋白在大肠杆菌中的高效表达与纯化》**
- 作者：张伟等
- 摘要：研究通过基因工程构建人LCN12重组质粒，在大肠杆菌中诱导表达并优化纯化条件，获得高纯度蛋白，经Western blot验证其免疫活性，为后续功能研究奠定基础。
2. **《LCN12在小鼠脂肪代谢中的调控作用》**
- 作者：Li, X. 等
- 摘要：利用重组LCN12蛋白处理小鼠脂肪细胞，发现其通过结合脂肪酸转运蛋白促进脂质积累，提示LCN12可能在肥胖相关代谢疾病中起关键作用。
3. **《LCN12与视黄醇结合蛋白的结构功能关系研究》**
- 作者：Wang, Y. 等
- 摘要：通过重组表达LCN12并解析其晶体结构，发现其具有典型脂质运载蛋白家族的β-桶结构，且与视黄醇结合能力相关，为靶向药物设计提供依据。
4. **《LCN12重组蛋白在炎症反应中的免疫调节功能》**
- 作者：Chen, J. 等
- 摘要：体外实验表明，重组LCN12可抑制巨噬细胞中促炎因子TNF-α的释放，暗示其在抗炎治疗中的潜在应用价值。
**注意**：以上内容为基于LCN12常见研究方向的模拟文献，实际文献需通过学术数据库(如PubMed、Google Scholar)以“LCN12 recombinant protein”“Lipocalin 12 function”等关键词检索确认。
**Background of LCN12 Recombinant Protein**
LCN12 (Lipocalin 12) is a member of the lipocalin protein family, characterized by its ability to bind and transport small hydrophobic molecules. This secretory protein is encoded by the *LCN12* gene and is primarily expressed in tissues such as the epididymis, liver, and certain epithelial cells. Lipocalins play roles in diverse biological processes, including immune regulation, cell signaling, and metabolic homeostasis. LCN12. in particular, has garnered interest due to its potential involvement in lipid metabolism, cellular communication, and interactions with cell surface receptors.
Recombinant LCN12 is produced using genetic engineering techniques, where the *LCN12* gene is cloned into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to enable large-scale protein synthesis. The recombinant form retains the native protein's structural and functional properties, including its characteristic eight-stranded β-barrel structure that forms a ligand-binding pocket. This allows researchers to study its interactions with metabolites, hormones, or signaling molecules in vitro.
Studies suggest that LCN12 may influence metabolic pathways, such as fatty acid uptake or steroid transport, and could serve as a biomarker for metabolic disorders. Its role in male reproductive health, particularly in sperm maturation, is also under investigation. Additionally, recombinant LCN12 has applications in structural biology for elucidating 3D conformations and in drug discovery for targeting metabolic diseases.
Despite progress, the full scope of LCN12's physiological roles remains unclear, necessitating further research into its binding partners, regulatory mechanisms, and tissue-specific functions. Recombinant protein technology continues to be pivotal in advancing these studies, offering tools for therapeutic development and mechanistic insights into lipocalin-mediated processes.
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