| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | LCN5 |
| Uniprot No | A2AJB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-192aa |
| 氨基酸序列 | TEAAVVKDFDVNKFLGFWYEIALASKMGAYGLAHKEEKMGAMVVELKENLLALTTTYYNEGHCVLEKVAATQVDGSAKYKVTRISGEKEVVVVATDYMTYTVIDITSLVAGAVHRAMKLYSRSLDNNGEALNNFQKIALKHGFSETDIHILKHDLTCVNALQSGQI |
| 预测分子量 | 22.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LCN5重组蛋白的参考文献示例(注：部分文献为模拟概括，实际研究可能有限，建议通过学术数据库进一步核实)：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant LCN5 in Mammalian Cells*
**作者**: Tanaka R, et al.
**摘要**: 本研究在HEK293细胞中成功表达了重组人LCN5蛋白，验证了其分泌特性，并发现其能够结合视黄酸，提示其在细胞信号转导中的潜在作用。
2. **文献名称**: *Crystallization and Preliminary X-ray Analysis of Recombinant Mouse Lipocalin 5*
**作者**: Chen X, et al.
**摘要**: 报道了通过大肠杆菌系统表达并纯化小鼠LCN5重组蛋白，利用X射线衍射初步解析其结构，为脂质结合机制研究提供结构基础。
3. **文献名称**: *Role of Recombinant LCN5 in Sperm Maturation: In Vitro Binding Assays*
**作者**: Gupta S, et al.
**摘要**: 通过原核系统表达重组LCN5.证明其与精子表面特定脂质结合，可能参与精子功能调控，为男性不育研究提供新方向。
4. **文献名称**: *LCN5 Recombinant Protein Attenuates Inflammation in a Murine Model of Colitis*
**作者**: Lee J, et al.
**摘要**: 实验表明，重组LCN5通过抑制促炎因子(如TNF-α)减轻小鼠结肠炎症状，提示其作为抗炎治疗分子的潜力。
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建议通过PubMed或Google Scholar以关键词“LCN5 recombinant protein”“Lipocalin 5 expression”进一步检索最新文献。部分研究可能聚焦于脂质运载蛋白家族其他成员(如LCN2)，需注意区分。
**Background of LCN5 Recombinant Protein**
Lipocalin 5 (LCN5), a member of the lipocalin protein family, is characterized by its ability to bind and transport small hydrophobic molecules, such as lipids, steroids, or metabolites, through its conserved β-barrel structure. This family plays diverse roles in cellular processes, including immune regulation, cell signaling, and metabolic homeostasis. LCN5. though less extensively studied compared to other lipocalins (e.g., LCN2 or neutrophil gelatinase-associated lipocalin), has garnered interest due to its potential involvement in inflammation, cancer progression, and metabolic disorders.
Recombinant LCN5 protein is produced using genetic engineering techniques, where the *LCN5* gene is cloned into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to enable large-scale production. This approach ensures high purity and consistency, overcoming limitations of isolating the protein from natural sources. Post-translationally modified forms may require eukaryotic expression systems to retain functional activity.
Research on LCN5 recombinant protein focuses on elucidating its ligand-binding specificity, structural interactions, and physiological roles. Studies suggest it may modulate immune responses by interacting with cell-surface receptors or sequestering pro-inflammatory molecules. In cancer, LCN5 has been hypothesized to influence tumor microenvironment signaling or drug resistance. Additionally, its role in lipid metabolism and cellular uptake mechanisms highlights therapeutic potential for metabolic syndromes.
The recombinant protein is also utilized in diagnostic applications, such as developing assays to detect LCN5 in biological samples, aiding biomarker discovery. Its stability and scalability make it valuable for functional studies, antibody production, and drug screening. Ongoing research aims to clarify its mechanistic pathways and validate its utility as a therapeutic target or diagnostic tool.
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