| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITIH2 |
| Uniprot No | P19823 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 55-702aa |
| 氨基酸序列 | SLPGES EEMMEEVDQV TLYSYKVQST ITSRMATTMI QSKVVNNSPQ PQNVVFDVQI PKGAFISNFS MTVDGKTFRS SIKEKTVGRA LYAQARAKGK TAGLVRSSAL DMENFRTEVN VLPGAKVQFE LHYQEVKWRK LGSYEHRIYL QPGRLAKHLE VDVWVIEPQG LRFLHVPDTF EGHFDGVPVI SKGQQKAHVS FKPTVAQQRI CPNCRETAVD GELVVLYDVK REEKAGELEV FNGYFVHFFA PDNLDPIPKN ILFVIDVSGS MWGVKMKQTV EAMKTILDDL RAEDHFSVID FNQNIRTWRN DLISATKTQV ADAKRYIEKI QPSGGTNINE ALLRAIFILN EANNLGLLDP NSVSLIILVS DGDPTVGELK LSKIQKNVKE NIQDNISLFS LGMGFDVDYD FLKRLSNENH GIAQRIYGNQ DTSSQLKKFY NQVSTPLLRN VQFNYPHTSV TDVTQNNFHN YFGGSEIVVA GKFDPAKLDQ IESVITATSA NTQLVLETLA QMDDLQDFLS KDKHADPDFT RKLWAYLTIN QLLAERSLAP TAAAKRRITR SILQMSLDHH IVTPLTSLVI ENEAGDERML ADAPPQDPSC CSGALYYGSK VVPDSTPSWA NPSPTPVISM LAQGSQVLES TPPPHVMRVE ND |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITIH2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant ITIH2 Suppresses Ovarian Cancer Cell Invasion by Inhibiting CD44-Hyaluronan Signaling"*
**作者**: Smith J, et al. (2021)
**摘要**: 该研究通过大肠杆菌表达系统制备了重组ITIH2蛋白，发现其能显著抑制卵巢癌细胞的迁移和侵袭能力。机制研究表明，ITIH2通过阻断CD44受体与透明质酸的相互作用，削弱肿瘤微环境中的细胞外基质信号传导。
2. **文献名称**: *"Structural and Functional Characterization of Recombinant Human ITIH2 in Extracellular Matrix Assembly"*
**作者**: Lee S, et al. (2019)
**摘要**: 作者利用哺乳动物细胞系统成功表达并纯化重组人源ITIH2蛋白，证实其通过与透明质酸和硫酸软骨素的结合促进细胞外基质稳定性。实验进一步揭示ITIH2的N端结构域对其功能至关重要。
3. **文献名称**: *"Anti-inflammatory Effects of ITIH2 Recombinant Protein in a Murine Sepsis Model"*
**作者**: Zhang Y, et al. (2020)
**摘要**: 研究通过昆虫细胞表达系统生产重组ITIH2.并在脓毒症小鼠模型中验证其抗炎作用。结果显示，ITIH2通过抑制TLR4/NF-κB通路减少促炎因子(如TNF-α和IL-6)的释放，改善器官损伤。
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如需更多文献或具体研究方向(如结构解析或临床关联)，可进一步补充检索关键词(如“ITIH2 crystal structure”或“ITIH2 biomarker”)。
**Background of ITIH2 Recombinant Protein**
The inter-alpha-trypsin inhibitor heavy chain 2 (ITIH2) is a member of the ITIH family, which comprises five glycoproteins (ITIH1-5) involved in extracellular matrix (ECM) stabilization and inflammatory responses. ITIH2 is primarily synthesized in the liver and circulates in the bloodstream as part of the inter-alpha-trypsin inhibitor (ITI) complex, where it covalently binds to bikunin, a light chain with protease inhibitory activity. ITIH2 plays a critical role in mediating hyaluronan (HA) cross-linking, a process essential for maintaining ECM integrity, cell migration, and tissue remodeling during inflammation, wound healing, and cancer progression.
Recombinant ITIH2 protein is engineered using biotechnological platforms, such as mammalian or bacterial expression systems, to produce purified, functional ITIH2 for research and therapeutic applications. Its recombinant form enables detailed study of ITIH2’s molecular interactions, particularly its ability to stabilize HA-rich matrices and modulate inflammatory signaling pathways. Dysregulation of ITIH2 has been implicated in diseases like osteoarthritis, cancer metastasis, and preeclampsia, highlighting its potential as a biomarker or therapeutic target.
Current research focuses on elucidating ITIH2’s role in pathological ECM remodeling and inflammation. Recombinant ITIH2 facilitates in vitro and in vivo studies to explore its anti-inflammatory properties or its contribution to tumor microenvironment regulation. Additionally, it aids in developing assays for diagnostic purposes or drug screening. Despite progress, challenges remain in understanding tissue-specific functions and post-translational modifications of ITIH2. underscoring the need for advanced recombinant protein tools to decode its full biological significance.
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