| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITIH1 |
| Uniprot No | P19827 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-911aa |
| 氨基酸序列 | MDGAMGPRGLLLCMYLVSLLILQAMPALGSATGRSKSSEKRQAVDTAVDG VFIRSLKVNCKVTSRFAHYVVTSQVVNTANEAREVAFDLEIPKTAFISDF AVTADGNAFIGDIKDKVTAWKQYRKAAISGENAGLVRASGRTMEQFTIHL TVNPQSKVTFQLTYEEVLKRNHMQYEIVIKVKPKQLVHHFEIDVDIFEPQ GISKLDAQASFLPKELAAQTIKKSFSGKKGHVLFRPTVSQQQSCPTCSTS LLNGHFKVTYDVSRDKICDLLVANNHFAHFFAPQNLTNMNKNVVFVIDIS GSMRGQKVKQTKEALLKILGDMQPGDYFDLVLFGTRVQSWKGSLVQASEA NLQAAQDFVRGFSLDEATNLNGGLLRGIEILNQVQESLPELSNHASILIM LTDGDPTEGVTDRSQILKNVRNAIRGRFPLYNLGFGHNVDFNFLEVMSME NNGRAQRIYEDHDATQQLQGFYSQVAKPLLVDVDLQYPQDAVLALTQNHH KQYYEGSEIVVAGRIADNKQSSFKADVQAHGEGQEFSITCLVDEEEMKKL LRERGHMLENHVERLWAYLTIQELLAKRMKVDREVRANLSSQALRMSLDY GFVTPLTSMSIRGMADQDGLKPTIDKPSEDSPPLEMLGPRRTFVLSALQP SPTHSSSNTQRLPDRVTGVDTDPHFIIHVPQKEDTLCFNINEEPGVILSL VQDPNTGFSVNGQLIGNKARSPGQHDGTYFGRLGIANPATDFQLEVTPQN ITLNPGFGGPVFSWRDQAVLRQDGVVVTINKKRNLVVSVDDGGTFEVVLH RVWKGSSVHQDFLGFYVLDSHRMSARTHGLLGQFFHPIGFEVSDIHPGSD PTKPDATMVVRNRRLTVTRGLQKDYSKDPWHGAEVSCWFIHNNGAGLIDG AYTDYIVPDIF |
| 预测分子量 | 126 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITIH1重组蛋白的3篇参考文献及其摘要概括：
1. **"Recombinant human inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1) inhibits ovarian cancer cell invasion by regulating hyaluronan metabolism"**
- 作者：Zou, L. et al.
- 摘要：研究通过重组表达ITIH1蛋白，发现其通过调控透明质酸代谢抑制卵巢癌细胞侵袭，揭示ITIH1在肿瘤微环境中的潜在抑癌作用。
2. **"Structural and functional characterization of recombinant ITIH1: Role in liver extracellular matrix stabilization"**
- 作者：Chen, Y. et al.
- 摘要：报道ITIH1重组蛋白的哺乳动物细胞表达系统构建及纯化，证实其通过与透明质酸和硫酸软骨素结合维持肝脏细胞外基质稳定性，为肝纤维化研究提供依据。
3. **"ITIH1 acts as a protease inhibitor: In vitro evidence from recombinant protein studies"**
- 作者：Lim, R. et al.
- 摘要：利用大肠杆菌系统表达重组ITIH1.证明其通过抑制基质金属蛋白酶(MMP-9)活性参与炎症调控，为炎症性疾病治疗提供新靶点。
注：以上文献为示例性质，实际引用时需根据具体研究内容核实原文。若需精准文献，建议通过PubMed或Web of Science以关键词“ITIH1 recombinant”检索最新研究。
**Background of ITIH1 Recombinant Protein**
Inter-alpha-trypsin inhibitor heavy chain 1 (ITIH1) is a glycoprotein component of the inter-alpha-trypsin inhibitor (ITI) family, primarily synthesized in the liver and secreted into plasma. ITIH1 plays a critical role in stabilizing the extracellular matrix (ECM) by covalently binding to hyaluronan (HA) and other matrix components via a process involving tumor necrosis factor-stimulated gene-6 (TSG-6). This interaction is vital for maintaining tissue integrity during inflammatory responses, wound healing, and ovulation. Dysregulation of ITIH1 has been linked to pathologies such as cancer metastasis, inflammatory disorders, and cardiovascular diseases.
Recombinant ITIH1 protein is engineered to mimic the native protein’s structure and function, enabling researchers to study its biological roles *in vitro* and *in vivo*. Produced using expression systems like mammalian cells (e.g., HEK293 or CHO), the recombinant form retains post-translational modifications essential for activity, such as glycosylation. It typically includes tags (e.g., His-tag) for purification and detection.
Studies utilizing ITIH1 recombinant protein have elucidated its anti-inflammatory properties, ECM remodeling mechanisms, and potential as a biomarker for diseases like hepatocellular carcinoma. Its ability to modulate HA-rich matrices also positions it as a therapeutic candidate for conditions involving abnormal ECM dynamics. However, challenges remain in understanding its isoform-specific functions and interactions with partner proteins. Overall, ITIH1 recombinant protein serves as a valuable tool for dissecting its physiological and pathological roles, offering insights for diagnostic and therapeutic advancements.
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