| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCN9 |
| Uniprot No | Q8WX39 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-176aa |
| 氨基酸序列 | QEFDP HTVMQRNYNV ARVSGVWYSI FMASDDLNRI KENGDLRVFV RNIEHLKNGS LIFDFEYMVQ GECVAVVVVC EKTEKNGEYS INYEGQNTVA VSETDYRLFI TFHLQNFRNG TETHTLALYE TCEKYGLGSQ NIIDLTNKDP CYSKHYRSPP RPPMRW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为根据LCN9相关研究领域构造的示例参考文献(请注意，这些文献信息为模拟示例，实际文献需通过学术数据库验证)：
1. **文献名称**: "Expression and Functional Analysis of Recombinant LCN9 Protein in Mammalian Cells"
**作者**: Zhang Y, et al.
**摘要**: 本研究成功构建了LCN9重组蛋白的真核表达系统，并验证其在哺乳动物细胞中的分泌表达。通过体外实验发现，重组LCN9可能通过调节脂质代谢参与精子成熟过程。
2. **文献名称**: "LCN9 Recombinant Protein Attenuates Inflammation in a Mouse Model of Sepsis"
**作者**: Kim S, Lee JH.
**摘要**: 利用大肠杆菌系统表达纯化LCN9重组蛋白，发现其在小鼠脓毒症模型中通过结合炎症因子(如IL-6)显著降低全身炎症反应，提示其作为抗炎治疗药物的潜力。
3. **文献名称**: "Structural Characterization of Human Lipocalin 9 and Its Role in Antimicrobial Defense"
**作者**: Müller R, et al.
**摘要**: 通过X射线晶体学解析了重组LCN9蛋白的三维结构，发现其与细菌铁载体具有高亲和力，体外实验证实其对革兰氏阴性菌的生长抑制作用，揭示了其在先天免疫中的功能。
4. **文献名称**: "LCN9 Recombinant Protein as a Potential Biomarker for Male Infertility: A Proteomic Study"
**作者**: Wang X, et al.
**摘要**: 基于精液蛋白质组学分析，发现LCN9在少精症患者中显著下调。通过重组蛋白功能回补实验，证实其缺失可能影响精子活力，提出其作为男性不育诊断标志物的可能性。
**建议验证途径**：
- 使用PubMed、Web of Science等平台，以关键词"LCN9 recombinant"或"lipocalin 9 expression"检索
- 结合基因别名(如"LCN9" OR "SIP27")扩大搜索范围
- 查阅脂质运载蛋白家族(lipocalin family)相关综述文献中的引用线索
Lipocalin-9 (LCN9), a member of the lipocalin protein family, is a small secretory protein implicated in diverse physiological processes, particularly in reproductive health and disease. Lipocalins are characterized by their conserved eight-stranded β-barrel structure, enabling binding and transport of hydrophobic molecules like lipids, steroids, or pheromones. LCN9 shares this structural framework but exhibits unique tissue-specific expression patterns, with notable prominence in reproductive tissues such as the epididymis, testes, and ovaries. Its expression is hormonally regulated, suggesting roles in gamete maturation, fertilization, or embryonic development.
Recombinant LCN9 refers to the protein produced via genetic engineering in heterologous systems (e.g., E. coli, mammalian cells), allowing controlled study of its biochemical and functional properties. Studies indicate LCN9 may interact with cell surface receptors or immune mediators, though its precise ligands and signaling mechanisms remain under investigation. Emerging evidence links LCN9 to pathological conditions, including male infertility and cancers. For instance, aberrant LCN9 levels correlate with sperm dysfunction, while its overexpression in certain tumors hints at roles in cell proliferation or metastasis.
Therapeutic and diagnostic applications drive interest in recombinant LCN9. Its potential as a biomarker for reproductive disorders or cancer is being explored, alongside engineered variants for targeted drug delivery. However, challenges persist in optimizing recombinant production yields, stability, and functional fidelity. Current research focuses on resolving its 3D structure, ligand-binding specificity, and in vivo pathways using recombinant protein tools. As a relatively understudied lipocalin, LCN9 represents a promising frontier for understanding reproductive biology and developing precision biotherapeutics.
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