| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCNT |
| Uniprot No | Q9BW27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656aa |
| 氨基酸序列 | MEELDGEPTVTLIPGVNSKKNQMYFDWGPGEMLVCETSFNKKEKSEMVPS CPFIYIIRKDVDVYSQILRKLFNESHGIFLGLQRIDEELTGKSRKSQLVR VSKNYRSVIRACMEEMHQVAIAAKDPANGRQFSSQVSILSAMELIWNLCE ILFIEVAPAGPLLLHLLDWVRLHVCEVDSLSADVLGSENPSKHDSFWNLV TILVLQGRLDEARQMLSKEADASPASAGICRIMGDLMRTMPILSPGNTQT LTELELKWQHWHEECERYLQDSTFATSPHLESLLKIMLGDEAALLEQKEL LSNWYHFLVTRLLYSNPTVKPIDLHYYAQSSLDLFLGGESSPEPLDNILL AAFEFDIHQVIKECSIALSNWWFVAHLTDLLDHCKLLQSHNLYFGSNMRE FLLLEYASGLFAHPSLWQLGVDYFDYCPELGRVSLELHIERIPLNTEQKA LKVLRICEQRQMTEQVRSICKILAMKAVRNNRLGSALSWSIRAKDAAFAT LVSDRFLRDYCERGCFSDLDLIDNLGPAMMLSDRLTFLGKYREFHRMYGE KRFADAASLLLSLMTSRIAPRSFWMTLLTDALPLLEQKQVIFSAEQTYEL MRCLEDLTSRRPVHGESDTEQLQDDDIETTKVEMLRLSLARNLARAIIRE GSLEGS |
| 预测分子量 | 98 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCNT(Pericentrin)重组蛋白的3篇参考文献及其摘要内容的简要概括：
1. **文献名称**：*Pericentrin, a highly conserved centrosome protein involved in microtubule organization*
**作者**：Doxsey, S. et al.
**摘要**：该研究首次报道了PCNT作为中心体核心蛋白的功能，阐明了其通过重组蛋白技术验证的微管组织能力，并揭示了其在维持中心体结构完整性中的作用。
2. **文献名称**：*Mutations in pericentrin cause dwarfism and disrupt microtubule dynamics*
**作者**：Rauch, A. et al.
**摘要**：作者通过重组PCNT蛋白的体外实验，证实其突变会导致微管锚定异常，进而解释与人类侏儒症(如Majewski综合征)相关的分子机制。
3. **文献名称**：*Recombinant pericentrin fragments reveal distinct binding domains for Plk1 and γ-tubulin*
**作者**：Matsuyama, M. et al.
**摘要**：研究利用重组PCNT蛋白片段进行互作实验，鉴定了其与激酶Plk1和γ-微管蛋白的结合区域，为解析中心体调控细胞周期的分子途径提供了依据。
4. **文献名称**：*Functional analysis of pericentrin isoforms using recombinant expression in mammalian cells*
**作者**：Flory, M.R. & Davis, T.N.
**摘要**：通过哺乳动物细胞表达系统生成重组PCNT异构体，揭示了不同剪接变体在中心体靶向定位和纤毛形成中的功能差异。
注：上述文献为示例，实际引用时需根据具体研究内容核实原文信息。
Pericentrin (PCNT), also known as kendrin, is a conserved centrosomal protein critical for maintaining centrosome integrity and regulating mitotic progression. It serves as a scaffolding component of the pericentriolar material (PCM), anchoring key regulatory proteins involved in microtubule nucleation, cell cycle control, and cilia formation. Structurally, PCNT contains coiled-coil domains and a conserved C-terminal PACT motif that mediates centrosome localization. Dysregulation of PCNT is linked to severe human diseases, including microcephalic osteodysplastic primordial dwarfism type II (MOPDII) and various cancers, underscoring its essential role in development and genomic stability.
Recombinant PCNT proteins are engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional PCNT for research. These proteins typically retain critical domains for interaction studies, such as binding sites for γ-tubulin, CDK5RAP2. or other PCM components. Tagged versions (e.g., His, GST, or FLAG tags) facilitate purification and detection. Applications include exploring centrosome dynamics, modeling disease-associated mutations, and screening therapeutic compounds targeting centrosome amplification in cancer. Recombinant PCNT also aids antibody development and structural studies via cryo-EM or X-ray crystallography. Its use overcomes limitations of native protein extraction, enabling precise mechanistic studies of centrosome-related pathologies. Current research focuses on how PCNT mutations disrupt mitotic fidelity and contribute to developmental disorders or tumorigenesis, highlighting its potential as a biomarker or therapeutic target.
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