纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | KCNN2 |
Uniprot No | Q9H2S1 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-579aa |
氨基酸序列 | MSSCRYNGGVMRPLSNLSASRRNLHEMDSEAQPLQPPASVGGGGGASSPSAAAAAAAAVSSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIEAQMESYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS |
预测分子量 | 63,7 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCNN2重组蛋白的3篇代表性文献的简要信息:
1. **文献名称**: "Functional characterization of recombinant human SK2 channels in mammalian cells"
**作者**: Weatherall, K.L. et al.
**摘要**: 本研究利用HEK293细胞成功表达重组人源KCNN2(SK2)通道蛋白,通过膜片钳技术证实其钙依赖性激活特性,并揭示其与钙调蛋白的动态相互作用机制。
2. **文献名称**: "Cryo-EM structure of the SK2-calmodulin complex in a calcium-bound state"
**作者**: Lee, C.H. & MacKinnon, R.
**摘要**: 通过冷冻电镜解析了重组表达的小鼠SK2通道与钙调蛋白复合体的高分辨率结构,阐明了钙离子结合后引起的构象变化及通道门控的分子基础。
3. **文献名称**: "Pharmacological modulation of recombinant SK2 channels: Implications for cognitive disorders"
**作者**: Pedarzani, P. et al.
**摘要**: 在CHO细胞中表达重组KCNN2蛋白,系统筛选了多种神经活性化合物对其电流的调控作用,发现特定激动剂可增强通道活性,为阿尔茨海默病治疗提供潜在靶点。
注:以上为模拟文献案例,实际引用时建议通过PubMed等数据库检索最新研究(如关键词:KCNN2 recombinant expression/SK2 channel purification)。
KCNN2 recombinant protein is derived from the potassium calcium-activated channel subfamily N member 2 (KCNN2), a gene encoding the small-conductance calcium-activated potassium (SK) channel SK2. These channels are voltage-independent and activated by intracellular calcium ions, playing critical roles in regulating cellular excitability, calcium signaling, and neurotransmitter release. The KCNN2/SK2 channel is predominantly expressed in the central nervous system, heart, and smooth muscle, where it contributes to afterhyperpolarization (AHP) phases of action potentials, thereby modulating neuronal firing patterns and cardiac rhythm.
Recombinant KCNN2 protein is typically produced using heterologous expression systems, such as mammalian cells (e.g., HEK293) or insect cells, to ensure proper post-translational modifications and functional folding. The protein is often engineered with affinity tags (e.g., His-tag, FLAG-tag) for purification and detection. Structurally, it comprises six transmembrane domains with intracellular N- and C-termini, forming a tetrameric pore selective for potassium ions. Its activation relies on calmodulin binding in response to elevated cytosolic calcium levels.
Research applications of KCNN2 recombinant protein include in vitro electrophysiological studies (e.g., patch-clamp), calcium sensitivity assays, and drug screening for neurological or cardiovascular disorders. Dysregulation of SK2 channels is implicated in epilepsy, ataxia, arrhythmias, and hypertension, making this protein a target for therapeutic development. Additionally, it aids in structural studies (e.g., cryo-EM) to elucidate channel gating mechanisms and interactions with modulators like positive allosteric activators or inhibitors. The recombinant form provides a controlled, scalable tool for mechanistic investigations and translational research.
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