| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FFAR2 |
| Uniprot No | O15552 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330aa |
| 氨基酸序列 | MLPDWKSSLILMAYIIIFLTGLPANLLALRAFVGRIRQPQPAPVHILLLSLTLADLLLLLLLPFKIIEAASNFRWYLPKVVCALTSFGFYSSIYCSTWLLAGISIERYLGVAFPVQYKLSRRPLYGVIAALVAWVMSFGHCTIVIIVQYLNTTEQVRSGNEITCYENFTDNQLDVVLPVRLELCLVLFFIPMAVTIFCYWRFVWIMLSQPLVGAQRRRRAVGLAVVTLLNFLVCFGPYNVSHLVGYHQRKSPWWRSIAVVFSSLNASLDPLLFYFSSSVVRRAFGRGLQVLRNQGSSLLGRRGKDTAEGTNEDRGVGQGEGMPSSDFTTE |
| 预测分子量 | 37,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FFAR2重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Structural basis for the ligand recognition of the free fatty acid receptor 2*
**作者**：Lu et al. (2022)
**摘要**：该研究通过昆虫细胞表达系统制备了人源FFAR2重组蛋白，并利用冷冻电镜技术解析了其与丙酸结合的复合物结构，揭示了短链脂肪酸与受体的结合模式及信号传导机制。
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2. **文献名称**：*Functional characterization of recombinant FFAR2/GPR43 in inflammatory responses*
**作者**：Kim et al. (2020)
**摘要**：作者在哺乳动物细胞中重组表达了FFAR2蛋白，发现其通过激活下游MAPK/ERK信号通路调控巨噬细胞的炎症反应，强调了FFAR2在代谢-免疫交叉调控中的作用。
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3. **文献名称**：*Expression and purification of FFAR2 for pharmacological profiling*
**作者**：Smith et al. (2018)
**摘要**：研究优化了FFAR2在大肠杆菌中的重组表达和纯化流程，结合表面等离子体共振(SPR)技术，筛选出多个高亲和力配体，为靶向FFAR2的药物开发提供实验基础。
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4. **文献名称**：*Short-chain fatty acids activate FFAR2 to regulate glucagon-like peptide-1 secretion*
**作者**：Tolhurst et al. (2012)
**摘要**：通过重组FFAR2蛋白模型，验证了短链脂肪酸(如乙酸)激活FFAR2的能力，并证明其在肠道L细胞中通过该受体促进GLP-1分泌，进而影响糖代谢稳态。
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以上文献涵盖了FFAR2重组蛋白的结构解析、功能验证、表达纯化及生理机制研究，可作为相关领域研究的参考。
Free Fatty Acid Receptor 2 (FFAR2), also known as GPR43. is a G protein-coupled receptor (GPCR) that plays a critical role in sensing short-chain fatty acids (SCFAs) like acetate, propionate, and butyrate. These SCFAs are primarily produced by gut microbiota through the fermentation of dietary fiber, linking FFAR2 to metabolic and immune regulation. FFAR2 is expressed in various tissues, including immune cells, adipose tissue, and intestinal endocrine cells, where it modulates inflammation, energy homeostasis, and hormone secretion. Its activation influences pathways such as Gi/o and Gq, triggering downstream signaling cascades that regulate cellular responses.
Recombinant FFAR2 protein is engineered using genetic techniques to produce purified, functional receptor proteins in heterologous systems like mammalian or bacterial cells. This enables researchers to study FFAR2's structure, ligand interactions, and signaling mechanisms in controlled environments. Recombinant forms are critical for high-throughput drug screening, structural biology (e.g., crystallography or cryo-EM), and functional assays to identify agonists or antagonists.
Research on FFAR2 highlights its therapeutic potential in metabolic disorders (e.g., obesity, diabetes), inflammatory diseases (e.g., colitis), and gut-microbiota interactions. Dysregulation of FFAR2 is linked to impaired SCFA signaling, contributing to metabolic inflammation and insulin resistance. By leveraging recombinant FFAR2. scientists aim to develop targeted therapies that mimic or block SCFA effects, offering novel approaches to modulate host-microbiome communication and treat associated conditions. Its role in immune cell activation and gut health further underscores its relevance in translational medicine.
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