纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CRY1 |
Uniprot No | Q16526 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-586aa |
氨基酸序列 | MGVNAVHWFRKGLRLHDNPALKECIQGADTIRCVYILDPWFAGSSNVGIN RWRFLLQCLEDLDANLRKLNSRLFVIRGQPADVFPRLFKEWNITKLSIEY DSEPFGKERDAAIKKLATEAGVEVIVRISHTLYDLDKIIELNGGQPPLTY KRFQTLISKMEPLEIPVETITSEVIEKCTTPLSDDHDEKYGVPSLEELGF DTDGLSSAVWPGGETEALTRLERHLERKAWVANFERPRMNANSLLASPTG LSPYLRFGCLSCRLFYFKLTDLYKKVKKNSSPPLSLYGQLLWREFFYTAA TNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQE GWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWL SCSSFFQQFFHCYCPVGFGRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA PEGIQKVAKCLIGVNYPKPMVNHAEASRLNIERMKQIYQQLSRYRGLGLL ASVPSNPNGNGGFMGYSAENIPGCSSSGSCSQGSGILHYAHGDSQQTHLL KQGRSSMGTGLSGGKRPSQEEDTQSIGPKVQRQSTN |
预测分子量 | 90 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRY1重组蛋白的3篇参考文献示例(注:文献信息为示例性内容,可能不对应真实文献):
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1. **文献名称**: *Expression and purification of recombinant human CRY1 protein in Escherichia coli*
**作者**: Smith A, Johnson B, Lee C
**摘要**: 本研究描述了利用大肠杆菌表达系统高效表达人源CRY1重组蛋白的优化方法,通过His标签亲和层析和离子交换层析纯化获得高纯度蛋白,并验证其与CLOCK/BMAL1复合物的体外结合活性。
2. **文献名称**: *Structural insights into the circadian function of CRY1 through recombinant protein crystallography*
**作者**: Takahashi S, et al.
**摘要**: 利用重组CRY1蛋白的晶体结构分析,揭示了其C端结构域在光周期调控中的关键作用,并阐明了CRY1与PER2蛋白相互作用的分子机制,为生物钟调控提供结构基础。
3. **文献名称**: *Functional characterization of recombinant CRY1 in DNA repair pathways*
**作者**: Patel R, et al.
**摘要**: 通过体外实验验证重组CRY1蛋白在UV诱导的DNA损伤修复中的功能,发现其与DNA修复酶XPA的互作,表明CRY1可能整合昼夜节律与DNA损伤应答。
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如需具体文献,建议在PubMed或Google Scholar中检索关键词“CRY1 recombinant protein expression”或“CRY1 purification and function”。
**Background of CRY1 Recombinant Protein**
Cryptochrome 1 (CRY1) is a member of the cryptochrome protein family, which plays a critical role in regulating circadian rhythms in mammals. As a core component of the molecular clock, CRY1 interacts with other clock proteins, such as CLOCK and BMAL1. to repress their transcriptional activity, thereby maintaining the 24-hour oscillatory cycle of gene expression. Beyond circadian regulation, CRY1 is involved in light-dependent and light-independent functions, including DNA repair and cellular stress responses.
Recombinant CRY1 protein is engineered through genetic engineering techniques, where the CRY1 gene is cloned into expression vectors and produced in heterologous systems like *E. coli* or mammalian cell cultures. This allows for large-scale production of purified, functional CRY1 protein for research and therapeutic applications.
The study of recombinant CRY1 has advanced our understanding of circadian biology and its links to diseases like sleep disorders, metabolic syndromes, and cancer. It serves as a tool for structural studies (e.g., crystallography), biochemical assays (e.g., protein-protein interaction analyses), and drug discovery targeting circadian rhythm-related pathologies. Additionally, recombinant CRY1 aids in exploring post-translational modifications (e.g., phosphorylation) that regulate its stability and activity.
Despite progress, challenges remain in elucidating CRY1's dual roles in circadian and non-circadian pathways. Research using recombinant CRY1 continues to uncover its molecular mechanisms, offering potential therapeutic strategies for disorders linked to clock dysregulation.
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