| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDH1 |
| Uniprot No | Q08174 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1060aa |
| 氨基酸序列 | MDSGAGGRRCPEAALLILGPPRMEHLRHSPGPGGQRLLLPSMLLALLLLLAPSPGHATRVVYKVPEEQPPNTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQLPGDPCILEFEVSITDLVQNGSPRLLEGQIEVQDINDNTPNFASPVITLAIPENTNIGSLFPIPLASDRDAGPNGVASYELQAGPEAQELFGLQVAEDQEEKQPQLIVMGNLDRERWDSYDLTIKVQDGGSPPRASSALLRVTVLDTNDNAPKFERPSYEAELSENSPIGHSVIQVKANDSDQGANAEIEYTFHQAPEVVRRLLRLDRNTGLITVQGPVDREDLSTLRFSVLAKDRGTNPKSARAQVVVTVKDMNDNAPTIEIRGIGLVTHQDGMANISEDVAEETAVALVQVSDRDEGENAAVTCVVAGDVPFQLRQASETGSDSKKKYFLQTTTPLDYEKVKDYTIEIVAVDSGNPPLSSTNSLKVQVVDVNDNAPVFTQSVTEVAFPENNKPGEVIAEITASDADSGSNAELVYSLEPEPAAKGLFTISPETGEIQVKTSLDREQRESYELKVVAADRGSPSLQGTATVLVNVLDCNDNDPKFMLSGYNFSVMENMPALSPVGMVTVIDGDKGENAQVQLSVEQDNGDFVIQNGTGTILSSLSFDREQQSTYTFQLKAVDGGVPPRSAYVGVTINVLDENDNAPYITAPSNTSHKLLTPQTRLGETVSQVAAEDFDSGVNAELIYSIAGGNPYGLFQIGSHSGAITLEKEIERRHHGLHRLVVKVSDRGKPPRYGTALVHLYVNETLANRTLLETLLGHSLDTPLDIDIAGDPEYERSKQRGNILFGVVAGVVAVALLIALAVLVRYCRQREAKSGYQAGKKETKDLYAPKPSGKASKGNKSKGKKSKSPKPVKPVEDEDEAGLQKSLKFNLMSDAPGDSPRIHLPLNYPPGSPDLGRHYRSNSPLPSIQLQPQSPSASKKHQVVQDLPPANTFVGTGDTTSTGSEQYSDYSYRTNPPKYPSKQVGQPFQLSTPQPLPHPYHGAIWTEVWE |
| 预测分子量 | 114,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **PCDH1重组蛋白** 的3篇参考文献示例(注：文献为虚拟示例，实际研究中请根据具体需求检索真实论文)：
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1. **标题**: *Recombinant PCDH1 Protein Production in Mammalian Cells and Its Role in Airway Epithelial Barrier Function*
**作者**: Smith A, et al.
**摘要**: 研究通过HEK293细胞表达重组PCDH1胞外域蛋白，发现其能增强上皮细胞间黏附，并减轻过敏原诱导的屏障破坏，提示其在哮喘治疗中的潜在应用。
2. **标题**: *Structural Characterization of PCDH1 Extracellular Domain Using Recombinant Protein Expressed in E. coli*
**作者**: Zhang Y, et al.
**摘要**: 利用大肠杆菌系统表达并纯化PCDH1胞外结构域重组蛋白，通过X射线晶体学解析其三维结构，揭示其钙离子依赖性同源结合机制。
3. **标题**: *PCDH1 Recombinant Protein Attenuates Viral-Induced Inflammation in Respiratory Syncytial Virus (RSV) Mouse Model*
**作者**: Johnson R, et al.
**摘要**: 在小鼠模型中，注射重组PCDH1蛋白可抑制RSV感染后炎症因子释放，表明其通过调节免疫反应减轻病毒感染相关肺损伤。
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**提示**：实际文献需通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词(如“PCDH1 recombinant protein”、“protocadherin-1 expression”)。关注研究重组蛋白表达系统(如哺乳动物细胞、昆虫细胞)、功能验证(细胞黏附、疾病模型)或结构分析的论文。
Protocadherin-1 (PCDH1), a member of the protocadherin family, is a calcium-dependent transmembrane protein primarily known for its role in cell-cell adhesion and signaling. This protein is structurally characterized by extracellular cadherin repeats containing calcium-binding motifs, a transmembrane domain, and cytoplasmic signaling motifs. PCDH1 is highly expressed in epithelial and endothelial tissues, where it contributes to tissue integrity, barrier formation, and intercellular communication. Dysregulation of PCDH1 has been implicated in respiratory diseases, particularly asthma, as genetic variants are linked to impaired lung function and airway hyperresponsiveness. It also interacts with viral pathogens, influencing infection mechanisms in conditions like bronchiolitis.
Recombinant PCDH1 protein, produced through engineered expression systems (e.g., mammalian CHO or HEK293 cells), retains functional domains essential for studying its biological activities. Purification methods often employ affinity tags (e.g., His-tag) followed by chromatography to ensure high purity and stability. This recombinant tool enables researchers to investigate PCDH1's role in disease pathways, screen therapeutic agents targeting its interactions, and develop diagnostic antibodies. Current research focuses on its dual role as a mediator of cell adhesion and a potential oncogenic factor in certain cancers, highlighting its therapeutic relevance. The development of recombinant PCDH1 has accelerated mechanistic studies, offering insights into its structural dynamics and ligand-binding properties, which are critical for designing targeted interventions in inflammatory and neoplastic disorders.
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