| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRM1 |
| Uniprot No | P04553 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-51aa |
| 氨基酸序列 | ARYRCCRSQ SRSRYYRQRQ RSRRRRRRSC QTRRRAMRCC RPRYRPRCRR H |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRM1重组蛋白的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**: *"Recombinant human protamine 1: Production and functional analysis in chromatin condensation assays"*
**作者**: Smith A, et al.
**摘要**: 研究报道了通过大肠杆菌系统成功表达并纯化重组人PRM1蛋白，验证了其在体外促进染色质凝聚的功能，并发现其与天然PRM1在结构和功能上高度相似。
2. **文献名称**: *"Optimization of Protamine 1 expression in yeast for studying sperm chromatin packaging"*
**作者**: Lee J, et al.
**摘要**: 在酵母系统中优化了重组PRM1的表达条件，证明其能够替代天然PRM1用于精子染色质包装机制研究，并揭示了其与DNA结合的动态特性。
3. **文献名称**: *"Structural and biochemical characterization of recombinant mouse PRM1 and its role in male fertility"*
**作者**: Chen X, et al.
**摘要**: 利用昆虫细胞系统制备重组小鼠PRM1蛋白，通过结构解析和转基因小鼠模型证实其在精子形成中的关键作用，为男性不育症研究提供分子基础。
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**备注**：以上文献为模拟示例，实际文献需通过PubMed、Web of Science等数据库检索关键词(如“PRM1 recombinant protein”或“recombinant protamine 1”)获取。若需具体文献，可进一步提供研究方向或数据库访问权限辅助筛选。
**Background of PRM1 Recombinant Protein**
Protamine 1 (PRM1), a small, arginine-rich nuclear protein, plays a critical role in sperm chromatin condensation during spermatogenesis. It replaces histones in late-stage spermatids, facilitating tight packaging of paternal DNA into a transcriptionally inert state, essential for genetic stability and successful fertilization. PRM1 dysfunction is linked to male infertility, abnormal sperm morphology, and DNA damage, highlighting its biological and clinical significance.
Recombinant PRM1 protein is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or eukaryotic expression systems. The process involves cloning the *PRM1* gene into a plasmid vector, followed by expression, purification (e.g., chromatography techniques), and validation (e.g., mass spectrometry, functional assays). Recombinant PRM1 retains DNA-binding activity, enabling studies on chromatin dynamics, nucleoprotamine structure, and infertility mechanisms.
Research applications include investigating PRM1’s role in epigenetic regulation, developing diagnostic tools for male reproductive disorders, and exploring its potential in gene delivery systems due to its DNA-condensing properties. Challenges include optimizing solubility (PRM1’s high arginine content complicates handling) and ensuring proper post-translational modifications in heterologous systems. Recent advances in protein engineering and CRISPR-based models continue to enhance its utility in reproductive biology and beyond.
Overall, PRM1 recombinant protein serves as a vital tool for unraveling sperm biology and advancing therapeutic strategies for infertility.
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