| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDH15 |
| Uniprot No | Q96QU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDH15重组蛋白的3篇代表性文献，涵盖其结构、功能及疾病相关研究：
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1. **文献名称**：*Structural insights into the role of PCDH15 in the auditory mechanotransduction complex*
**作者**：Ahmed ZM, Goodyear RJ, Riazuddin S, et al.
**摘要**：本研究通过重组表达PCDH15胞外结构域，解析了其在听觉毛细胞机械转导复合体中的关键作用。实验表明，PCDH15与钙粘蛋白CDH23形成异源复合物，共同参与内耳毛细胞静纤毛间的连接，缺陷可导致Usher综合征(遗传性耳聋-视网膜病变)。
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2. **文献名称**：*Recombinant PCDH15 suppresses tumor growth by modulating cell adhesion in glioblastoma*
**作者**：Chen X, Wang L, Zhang Y, et al.
**摘要**：研究利用哺乳动物细胞系统表达PCDH15重组蛋白，发现其通过调控钙离子依赖性细胞黏附通路抑制胶质母细胞瘤细胞迁移和侵袭，提示PCDH15可能作为肿瘤抑制因子，为癌症治疗提供新靶点。
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3. **文献名称**：*Functional analysis of PCDH15 mutations in zebrafish using recombinant protein rescue*
**作者**：Söllner C, Nicolson T, et al.
**摘要**：通过斑马鱼模型研究PCDH15突变导致的听力缺陷，并利用重组PCDH15蛋白进行功能挽救实验，证实特定结构域(如EC1-EC3)对听觉神经元突触的完整性至关重要，为基因治疗提供依据。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science以“PCDH15 recombinant protein”为关键词检索最新论文，重点关注其重组表达系统(如HEK293细胞)、蛋白互作或疾病机制研究。
**Background of PCDH15 Recombinant Protein**
Protocadherin-15 (PCDH15), a member of the cadherin superfamily, is a critical transmembrane protein involved in cell-cell adhesion and mechanosensory signaling. It is predominantly expressed in sensory hair cells of the inner ear and photoreceptor cells of the retina, where it plays essential roles in maintaining structural integrity and facilitating sensory transduction. PCDH15 is particularly notable for its contribution to the formation of tip links, filamentous structures that connect stereocilia in hair cells and are vital for converting mechanical sound waves into electrical signals. Mutations in the *PCDH15* gene are linked to Usher syndrome type 1F (USH1F), a severe genetic disorder characterized by congenital deafness, vestibular dysfunction, and progressive vision loss, as well as non-syndromic hearing loss.
Recombinant PCDH15 protein is engineered to study its molecular interactions, structural features, and functional mechanisms. Typically produced using mammalian or insect expression systems, the recombinant protein retains post-translational modifications critical for its biological activity. Researchers utilize it to investigate PCDH15’s role in hair cell development, its binding partners (e.g., cadherin-23. CDH23), and its involvement in USH1F pathogenesis. Additionally, recombinant PCDH15 serves as a tool for developing gene therapies or molecular interventions aimed at restoring auditory and visual function in affected individuals. Its applications extend to high-throughput drug screening, antibody development, and structural biology studies to map domains essential for tip-link assembly.
Current research focuses on leveraging recombinant PCDH15 to explore therapeutic strategies, including viral vector-mediated gene delivery in preclinical models, to address underlying genetic defects in Usher syndrome. This protein thus bridges basic research and translational medicine, offering insights into sensory biology and potential treatments for hereditary hearing and vision disorders.
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