| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ICA1 |
| Uniprot No | Q05084 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MSGHKCSYPWDLQDRYAQDKSVVNKMQQKYWETKQAFIKATGKKEDEHVVASDADLDAKLELFHSIQRTCLDLSKAIVLYQKRICFLSQEENELGKFLRSQGFQDKTRAGKMMQATGKALCFSSQQRLALRNPLCRFHQEVETFRHRAISDTWLTVNRMEQCRTEYRGALLWMKDVSQELDPDLYKQMEKFRKVQTQVRLAKKNFDKLKMDVCQKVDLLGASRCNLLSHMLATYQTTLLHFWEKTSHTMAAIHESFKGYQPYEFTTLKSLQDPMKKLVEKEEKKKINQQESTDAAVQEPSQLISLEEENQRKESSSFKTEDGKSILSALDKGSTHTACSGPIDELLDMKSEEGACLGPVAGTPEPEGADKDDLLLLSEIFNASSLEEGEFSKEWAAVFGDGQVKEPVPTMALGEPDPKAQTGSGFLPSQLLDQNMKDLQASLQEPAKAASDLTAWFSLFADLDPLSNPDAVGKTDKEHELLNA |
| 预测分子量 | 62.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ICA1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：Production of recombinant islet cell autoantigen 1 (ICA1) in E. coli for diabetes autoantibody detection
**作者**：Schlosser M, et al.
**摘要**：该研究描述了大肠杆菌中重组ICA1蛋白的高效表达与纯化，并验证其在1型糖尿病血清自身抗体检测中的应用，证实其与患者血清反应性高度相关。
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2. **文献名称**：Structural and functional analysis of ICA1 reveals its role in insulin secretion
**作者**：Wenzlau JM, et al.
**摘要**：通过重组ICA1蛋白的晶体结构解析，揭示了其与胰岛素分泌相关的分子机制，并发现其自身抗原表位与糖尿病自身免疫反应的关键关联。
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3. **文献名称**：Optimization of recombinant ICA1 protein for improved diagnostic sensitivity in autoimmune diabetes
**作者**：Achenbach P, et al.
**摘要**：研究改进了ICA1重组蛋白的表达条件及纯化策略，显著提升其在ELISA检测中的灵敏度和特异性，为临床早期诊断提供可靠工具。
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注：以上文献信息为示例性质，实际引用需核对真实数据库(如PubMed)并补充发表年份及期刊详情。
ICA1 (Islet Cell Autoantigen 1) is a protein encoded by the *ICA1* gene, primarily expressed in pancreatic islet β-cells and neuroendocrine tissues. It plays a role in membrane trafficking, insulin secretion, and synaptic vesicle dynamics. Structurally, ICA1 contains multiple functional domains, including a proline-rich region and a C-terminal PDZ-binding motif, enabling interactions with proteins involved in secretory pathways.
In type 1 diabetes (T1D), ICA1 is a major autoantigen targeted by autoreactive T cells and autoantibodies during autoimmune destruction of β-cells. Its immunogenicity has made it a key biomarker for studying T1D pathogenesis and diagnosis. Recombinant ICA1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains antigenic epitopes and is widely used in immunoassays (e.g., ELISA) to detect autoantibodies in patient sera.
Research applications include elucidating molecular mechanisms of β-cell dysfunction, validating autoimmune responses, and screening therapeutic agents. Additionally, recombinant ICA1 supports structural studies to map antibody-binding regions and engineer antigen-specific immunotherapies. Its role extends beyond diabetes, with emerging links to neurological disorders due to neuronal expression.
Overall, recombinant ICA1 serves as a critical tool in autoimmune research, bridging molecular biology, clinical diagnostics, and therapeutic development.
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