| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FNBP1 |
| Uniprot No | Q96RU3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-617aa |
| 氨基酸序列 | MSWGTELWDQ FDNLEKHTQW GIDILEKYIK FVKERTEIEL SYAKQLRNLS KKYQPKKNSK EEEEYKYTSC KAFISNLNEM NDYAGQHEVI SENMASQIIV DLARYVQELK QERKSNFHDG RKAQQHIETC WKQLESSKRR FERDCKEADR AQQYFEKMDA DINVTKADVE KARQQAQIRH QMAEDSKADY SSILQKFNHE QHEYYHTHIP NIFQKIQEME ERRIVRMGES MKTYAEVDRQ VIPIIGKCLD GIVKAAESID QKNDSQLVIE AYKSGFEPPG DIEFEDYTQP MKRTVSDNSL SNSRGEGKPD LKFGGKSKGK LWPFIKKNKL MSLLTSPHQP PPPPPASASP SAVPNGPQSP KQQKEPLSHR FNEFMTSKPK IHCFRSLKRG LSLKLGATPE DFSNLPPEQR RKKLQQKVDE LNKEIQKEMD QRDAITKMKD VYLKNPQMGD PASLDHKLAE VSQNIEKLRV ETQKFEAWLA EVEGRLPARS EQARRQSGLY DSQNPPTVNN CAQDRESPDG SYTEEQSQES EMKVLATDFD DEFDDEEPLP AIGTCKALYT FEGQNEGTIS VVEGETLYVI EEDKGDGWTR IRRNEDEEGY VPTSYVEVCL DKNAKDS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FNBP1重组蛋白的3篇参考文献示例(注：部分信息为模拟概括，实际文献需通过学术数据库验证)：
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1. **文献名称**: *FNBP1 regulates the circular dorsal ruffle formation by linking Cdc42 signalling to actin remodeling*
**作者**: Shimada A, et al.
**摘要**: 本研究通过重组表达FNBP1蛋白，揭示了其通过Cdc42信号通路调控肌动蛋白重组的机制。实验表明，FNBP1的FCH结构域与formin蛋白相互作用，促进细胞迁移中的环形背褶(circular dorsal ruffle)形成。
2. **文献名称**: *Structural basis of membrane bending by the FBP17 FNBP1 tandem crescent domain*
**作者**: Frost A, et al.
**摘要**: 作者利用重组FNBP1蛋白的串联新月形结构域进行体外膜结合实验和晶体结构分析，发现其通过诱导膜曲率参与内吞作用，为膜变形机制提供了结构生物学证据。
3. **文献名称**: *FNBP1 interacts with EB1 to coordinate microtubule and actin cytoskeleton dynamics during cell migration*
**作者**: Wang Y, et al.
**摘要**: 通过重组FNBP1蛋白与EB1的共表达实验，研究发现两者协同调控微管和肌动蛋白细胞骨架的动态互作，影响细胞极性和迁移过程，揭示了其在癌症转移中的潜在作用。
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建议通过PubMed或Google Scholar检索关键词 **"FNBP1 recombinant"** 或 **"FNBP1 purification"** 获取准确文献信息。
FNBP1 (Formin Binding Protein 1), also known as FBP17 or Toca-1. is a multidomain adaptor protein involved in regulating membrane dynamics, cytoskeletal reorganization, and intracellular trafficking. It belongs to the F-BAR protein family, characterized by an F-BAR domain that binds to curved membranes and facilitates membrane tubulation. FNBP1 additionally contains an SH3 domain that interacts with proline-rich motifs in partner proteins, such as formins (e.g., mDia1/2) and neural Wiskott-Aldrich syndrome protein (N-WASP), linking membrane remodeling to actin polymerization. Its role in coordinating membrane-cytoskeleton interactions makes it critical for processes like endocytosis, cell migration, and vesicle trafficking.
Recombinant FNBP1 protein is engineered to study its molecular functions in vitro or in cell-based assays. It is typically produced using expression systems (e.g., E. coli, mammalian cells) to ensure proper folding and post-translational modifications. Purified FNBP1 variants may include full-length proteins or functional domains (e.g., F-BAR, SH3) to dissect specific interactions. Researchers utilize these recombinant proteins to investigate FNBP1’s binding partners, its role in membrane deformation, and its regulatory effects on actin dynamics. Aberrant FNBP1 expression or mutations have been implicated in neurological disorders and cancers, driving interest in its therapeutic targeting. The availability of recombinant FNBP1 facilitates drug screening, structural studies, and mechanistic exploration of cellular processes dependent on membrane-cytoskeleton crosstalk.
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