| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLIN3 |
| Uniprot No | O60664 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-434aa |
| 氨基酸序列 | SADGAEADG STQVTVEEPV QQPSVVDRVA SMPLISSTCD MVSAAYASTK ESYPHIKTVC DAAEKGVRTL TAAAVSGAQP ILSKLEPQIA SASEYAHRGL DKLEENLPIL QQPTEKVLAD TKELVSSKVS GAQEMVSSAK DTVATQLSEA VDATRGAVQS GVDKTKSVVT GGVQSVMGSR LGQMVLSGVD TVLGKSEEWA DNHLPLTDAE LARIATSLDG FDVASVQQQR QEQSYFVRLG SLSERLRQHA YEHSLGKLRA TKQRAQEALL QLSQVLSLME TVKQGVDQKL VEGQEKLHQM WLSWNQKQLQ GPEKEPPKPE QVESRALTMF RDIAQQLQAT CTSLGSSIQG LPTNVKDQVQ QARRQVEDLQ ATFSSIHSFQ DLSSSILAQS RERVASAREA LDHMVEYVAQ NTPVTWLVGP FAPGITEKAP EEKK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLIN3重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**: "Recombinant Perilipin 3 modulates lipid droplet dynamics in live cells"
**作者**: Brasaemle DL, et al.
**摘要**: 该研究通过大肠杆菌表达系统制备了重组人源PLIN3蛋白，并利用荧光标记技术证明其在体外可结合人工脂滴表面，调节脂滴融合与分解的动态过程，揭示了PLIN3在细胞内脂代谢中的分子机制。
2. **文献名称**: "Structural characterization of recombinant human PLIN3 reveals phosphorylation-dependent membrane interactions"
**作者**: Saito K, et al.
**摘要**: 作者利用昆虫细胞表达系统获得高纯度PLIN3重组蛋白，结合圆二色谱和冷冻电镜分析其结构，发现其N端结构域的磷酸化修饰显著增强与脂膜的结合能力，为肥胖相关代谢疾病的治疗提供了结构基础。
3. **文献名称**: "PLIN3 recombinant protein attenuates hepatic steatosis via autophagy activation in murine models"
**作者**: Wang Y, et al.
**摘要**: 研究通过哺乳动物细胞表达的重组PLIN3蛋白处理非酒精性脂肪肝小鼠模型，发现其通过激活自噬通路促进肝细胞内脂滴降解，显著改善肝脏脂质堆积，提示PLIN3蛋白在代谢性疾病中的治疗潜力。
备注：上述文献信息为模拟示例，实际引用需以具体论文数据为准。建议通过PubMed或Web of Science以“PLIN3 recombinant”“Perilipin 3 expression”等关键词检索最新实证研究。
**Background of PLIN3 Recombinant Protein**
Perilipin 3 (PLIN3), also known as TIP47 (Tail-interacting protein of 47 kDa), is a member of the perilipin family of lipid droplet (LD)-associated proteins. These proteins play critical roles in lipid storage, metabolism, and LD dynamics by stabilizing the interface between neutral lipids and the aqueous cellular environment. PLIN3 is ubiquitously expressed and localizes to nascent or small LDs, where it regulates LD formation, maturation, and interactions with organelles such as the endoplasmic reticulum and mitochondria.
Recombinant PLIN3 protein is engineered to study its structural and functional roles in lipid metabolism. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains key domains, including the PAT domain (common to perilipin family members) and amphipathic helices, enabling lipid-binding and LD-targeting capabilities. Researchers use recombinant PLIN3 to investigate its interactions with lipases, chaperones (e.g., HSP70), and signaling molecules, as well as its involvement in metabolic disorders like obesity, diabetes, and atherosclerosis.
PLIN3’s role extends beyond lipid storage; it is implicated in cellular stress responses, autophagy, and cancer progression. Dysregulation of PLIN3 expression correlates with altered lipid accumulation in hepatosteatosis and cardiovascular diseases. Recombinant PLIN3 facilitates *in vitro* studies, including lipid-protein binding assays, cellular LD imaging, and mechanistic explorations of metabolic pathways. Its application also extends to drug discovery, targeting LD-associated pathologies.
In summary, PLIN3 recombinant protein serves as a vital tool for decoding lipid homeostasis mechanisms and their implications in health and disease, bridging molecular insights to therapeutic innovations.
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