| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAS2R38 |
| Uniprot No | P59533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333aa |
| 氨基酸序列 | MLTLTRIRTVSYEVRSTFLFISVLEFAVGFLTNAFVFLVNFWDVVKRQALSNSDCVLLCL SISRLFLHGLLFLSAIQLTHFQKLSEPLNHSYQAIIMLWMIANQANLWLAACLSLLYCSK LIRFSHTFLICLASWVSRKISQMLLGIILCSCICTVLCVWCFFSRPHFTVTTVLFMNNNT RLNWQIKDLNLFYSFLFCYLWSVPPFLLFLVSSGMLTVSLGRHMRTMKVYTRNSRDPSLE AHIKALKSLVSFFCFFVISSCAAFISVPLLILWRDKIGVMVCVGIMAACPSGHAAILISG NAKLRRAVMTILLWAQSSLKVRADHKADSRTLC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAS2R38重组蛋白的3篇参考文献及其摘要概括：
1. **Bufe, B., et al. (2005).**
**《The Molecular Basis of Individual Differences in Phenylthiocarbamide and Propylthiouracil Bitterness Perception》**
**摘要**：本研究通过重组表达TAS2R38受体，揭示了其两种常见单倍型(PAV和AVI)对苯硫脲(PTC)和丙硫氧嘧啶(PROP)苦味感知的差异，解释了人群中苦味敏感性的遗传变异。
2. **Sainz, E., et al. (2007).**
**《Functional Characterization of Human Bitter Taste Receptors》**
**摘要**：作者在HEK293细胞中重组表达了TAS2R38.利用钙离子成像技术系统分析了其对多种苦味化合物的反应，证实该受体对PTC和特定硫脲类化合物具有高度特异性。
3. **Marchiori, A., et al. (2020).**
**《Optimization of heterologous expression and purification of the human bitter taste receptor TAS2R38》**
**摘要**：该研究优化了在昆虫细胞系统中重组表达和纯化TAS2R38的条件，提高了蛋白产量和稳定性，为后续结构功能研究提供了可靠方法。
4. **Floriano, W.B., et al. (2006).**
**《Modeling the human PTC bitter-taste receptor polymorphisms predicts phenotypic variation in receptor activity》**
**摘要**：结合计算机模拟与重组受体功能实验，预测了TAS2R38不同突变体(如PAV/AVI)的活性差异，揭示了关键氨基酸残基在配体结合中的作用。
这些文献涵盖了TAS2R38重组蛋白的功能分析、表达优化及遗传多态性研究，反映了其在苦味感知机制中的重要性。
TAS2R38 is a bitter taste receptor belonging to the G protein-coupled receptor (GPCR) family, encoded by the TAS2R38 gene located on human chromosome 7. It plays a critical role in detecting bitter compounds, particularly phenylthiocarbamide (PTC) and propylthiouracil (PROP), which are structurally similar to toxins found in plants. Genetic polymorphisms in TAS2R38. such as single-nucleotide variants (e.g., A49P, V262A, I296V), give rise to distinct haplotypes (e.g., PAV, AVI), leading to individual differences in bitter taste perception. These variations divide populations into "tasters," "non-tasters," or "supertasters," influencing dietary preferences and nutrient intake.
Recombinant TAS2R38 protein is engineered in vitro using expression systems like HEK293 or insect cells to study its structure-function relationships, ligand specificity, and signaling mechanisms. This approach enables researchers to bypass challenges posed by low natural receptor abundance and tissue-specific expression. Studies using recombinant TAS2R38 have revealed its role beyond taste perception, including interactions with bacterial metabolites in airway innate immunity and potential links to metabolic disorders. Its characterization has also facilitated drug discovery, particularly in bitter-masking agents for pharmaceuticals and understanding bitter compound toxicity. However, functional variability due to haplotype-dependent receptor activity underscores the need for personalized approaches in applications ranging from nutrition to therapeutics. The recombinant protein thus serves as a vital tool for decoding bitter signaling pathways and their broader physiological implications.
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