| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLIN5 |
| Uniprot No | Q00G26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 氨基酸序列 | MSEEEAAQIPRSSVWEQDQQNVVQRVVALPLVRATCTAVCDVYSAAKDRHPLLGSACRLAENCVCGLTTRALDHAQPLLEHLQPQLATMNSLACRGLDKLEEKLPFLQQPSETVVTSAKDVVASSVTGVVDLARRGRRWSVELKRSVSHAVDVVLEKSEELVDHFLPMTEEELAALAAEAEGPEVGSVEDQRRQQGYFVRLGSLSARIRHLAYEHSVGKLRQSKHRAQDTLAQLQETLELIDHMQCGVTPTAPACPGKVHELWGEWGQRPPESRRRSQAELETLVLSRSLTQELQGTVEALESSVRGLPAGAQEKVAEVRRSVDALQTAFADARCFRDVPAAALAEGRGRVAHAHACVDELLELVVQAVPLPWLVGPFAPILVERPEPLPDLADLVDEVIGGPDPRWAHLDWPAQQRAWEAEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPELDF |
| 预测分子量 | 50,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLIN5重组蛋白的3篇参考文献概览：
1. **文献名称**：*Perilipin 5 restores lipid droplet degradation in myotubes from type 2 diabetic patients*
**作者**：Bosma, M., et al.
**摘要**：该研究利用重组PLIN5蛋白，在2型糖尿病患者骨骼肌细胞模型中验证了PLIN5通过促进脂滴水解和脂肪酸氧化改善胰岛素抵抗的作用机制。
2. **文献名称**：*Structural insights into the interaction of human PLIN5 with CGI-58 and its regulation by phosphorylation*
**作者**：Wang, H., et al.
**摘要**：通过重组表达人源PLIN5蛋白并结合X射线晶体学分析，揭示了PLIN5与激活因子CGI-58的相互作用结构域，并发现磷酸化修饰调控其脂解功能的关键位点。
3. **文献名称**：*Recombinant PLIN5 attenuates oxidative stress in cardiomyocytes by modulating lipid droplet-mitochondria coupling*
**作者**：Kimmel, A.R., Sztalryd, C.
**摘要**：研究证明重组PLIN5通过增强脂滴与线粒体的物理耦联，促进脂肪酸高效利用并减少活性氧积累，从而缓解心肌细胞氧化损伤。
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**备注**：以上文献为示例性质，具体内容需根据实际论文调整。建议通过PubMed或Web of Science以“PLIN5 recombinant protein”为关键词检索近期文献。
PLIN5 (Perilipin 5), a member of the perilipin family of lipid droplet-associated proteins, plays a critical role in lipid metabolism and cellular energy homeostasis. It is predominantly expressed in oxidative tissues such as the heart, liver, and skeletal muscle, where it regulates lipid storage and utilization. PLIN5 interacts with intracellular lipid droplets, coordinating the balance between lipolysis and fatty acid oxidation by modulating the activity of enzymes like adipose triglyceride lipase (ATGL) and its coactivator CGI-58. Its expression is upregulated under conditions of metabolic stress, such as fasting or exercise, and is transcriptionally regulated by peroxisome proliferator-activated receptor alpha (PPARα).
Recombinant PLIN5 protein is engineered using genetic recombination techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This protein retains functional domains essential for lipid droplet binding and protein-protein interactions, making it a valuable tool for studying lipid metabolism mechanisms in vitro. Researchers employ recombinant PLIN5 to investigate its role in metabolic diseases, including obesity, diabetes, and atherosclerosis, where dysregulated lipid storage contributes to pathogenesis. It also serves as a potential therapeutic target for modulating lipotoxicity in cardiomyocytes or hepatocytes.
The development of recombinant PLIN5 has advanced structural studies, enabling elucidation of its interaction interfaces with partners like PPARα or ATGL. Its high purity and specificity facilitate drug discovery efforts aimed at targeting lipid metabolism pathways. Overall, recombinant PLIN5 bridges basic research and translational applications, offering insights into metabolic disorders and guiding novel treatment strategies.
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