| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SYTL2 |
| Uniprot No | Q9HCH5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-934aa |
| 氨基酸序列 | MIDLSFLTEEEQEAIMKVLQRDAALKRAEEERVRHLPEKIKDDQQLKNMSGQWFYEAKAKRHRDKIHGADIIRASMRKKRPQIAAEQSKDRENGAKESWVNNVNKDAFLPPELAGVVEEPEEDAAPASPSSSVVNPASSVIDMSQENTRKPNVSPEKRKNPFNSSKLPEGHSSQQTKNEQSKNGRTGLFQTSKEDELSESKEKSTVADTSIQKLEKSKQTLPGLSNGSQIKAPIPKARKMIYKSTDLNKDDNQSFPRQRTDSLKARGAPRGILKRNSSSSSTDSETLRYNHNFEPKSKIVSPGLTIHERISEKEHSLEDNSSPNSLEPLKHVRFSAVKDELPQSPGLIHGREVGEFSVLESDRLKNGMEDAGDTEEFQSDPKPSQYRKPSLFHQSTSSPYVSKSETHQPMTSGSFPINGLHSHSEVLTARPQSMENSPTINEPKDKSSELTRLESVLPRSPADELSHCVEPEPSQVPGGSSRDRQQGSEEEPSPVLKTLERSAARKMPSKSLEDISSDSSNQAKVDNQPEELVRSAEDVSTVPTQPDNPFSHPDKLKRMSKSVPAFLQDESDDRETDTASESSYQLSRHKKSPSSLTNLSSSSGMTSLSSVSGSVMSVYSGDFGNLEVKGNIQFAIEYVESLKELHVFVAQCKDLAAADVKKQRSDPYVKAYLLPDKGKMGKKKTLVVKKTLNPVYNEILRYKIEKQILKTQKLNLSIWHRDTFKRNSFLGEVELDLETWDWDNKQNKQLRWYPLKRKTAPVALEAENRGEMKLALQYVPEPVPGKKLPTTGEVHIWVKECLDLPLLRGSHLNSFVKCTILPDTSRKSRQKTRAVGKTTNPIFNHTMVYDGFRPEDLMEACVELTVWDHYKLTNQFLGGLRIGFGTGKSYGTEVDWMDSTSEEVALWEKMVNSPNTWIEATLPLRMLLIAKISK |
| 预测分子量 | 104,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SYTL2重组蛋白的假设性参考文献示例(仅供格式参考，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Functional characterization of recombinant SYTL2 protein in exosome secretion"
**作者**: Zhang L, et al.
**摘要**: 研究通过重组SYTL2蛋白表达，证实其通过与Rab27A相互作用调控外泌体分泌，敲低SYTL2显著抑制HeLa细胞的外泌体释放。
2. **文献名称**: "Structural insights into the lipid-binding properties of SYTL2 C2 domain"
**作者**: Wang Y, et al.
**摘要**: 解析重组SYTL2蛋白的C2结构域晶体结构，揭示其对磷脂酸的特异性结合，为SYTL2参与膜运输机制提供结构基础。
3. **文献名称**: "SYTL2 promotes cancer cell invasion via regulating MMP-9 secretion"
**作者**: Chen X, et al.
**摘要**: 利用重组SYTL2蛋白进行功能实验，发现其通过激活ERK信号通路促进基质金属蛋白酶(MMP-9)分泌，增强乳腺癌细胞侵袭能力。
4. **文献名称**: "Development of a SYTL2 recombinant protein-based inhibitor for metastatic suppression"
**作者**: Kim S, et al.
**摘要**: 设计基于SYTL2重组蛋白的竞争性抑制剂，在体外和小鼠模型中显著抑制肿瘤细胞迁移和转移，提示其治疗潜力。
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**建议**：实际研究中，请通过PubMed、Google Scholar或Web of Science检索关键词“SYTL2 recombinant protein”“SYTL2 exocytosis”等获取真实文献。
**Background of SYTL2 Recombinant Protein**
SYTL2 (synaptotagmin-like protein 2), also known as exophilin-4 or Slp2. is a member of the synaptotagmin-like protein (Slp) family, which is characterized by conserved N-terminal Rab-binding domains and C-terminal C2 domains. This protein plays a critical role in intracellular vesicle trafficking and membrane dynamics, particularly in secretory pathways. SYTL2 interacts with Rab27A/B, small GTPases that regulate exocytosis and vesicle docking, facilitating processes like melanosome transport in melanocytes, hormone secretion in endocrine cells, and cytotoxic granule release in immune cells.
The recombinant SYTL2 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and functionality. Its recombinant form allows researchers to study SYTL2-Rab27 interactions, vesicle transport mechanisms, and its role in diseases such as Griscelli syndrome type 3 (linked to Rab27A mutations) or cancers with dysregulated secretion.
Studies using SYTL2 recombinant protein have provided insights into its structure-function relationships, including how its C2 domains mediate calcium-dependent membrane binding. Additionally, it serves as a tool for screening inhibitors targeting vesicle trafficking pathways, with potential therapeutic applications in immune disorders or metastatic cancers. Further research aims to unravel its tissue-specific roles and regulatory networks in cellular communication.
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