| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP2 |
| Uniprot No | P11137-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-471aa |
| 氨基酸序列 | MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPY REDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTA EAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVTVE EAAGGESALAPSVFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSG DRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTP PSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPAT PKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTS KCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVK IDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLL ESPQLATLAEDVTAALAKQGL |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP2重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要概述：
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1. **文献名称**：*"Recombinant MAP2c Protein Expression and Its Role in Microtubule Stabilization"*
**作者**：Thompson, R. et al. (1998)
**摘要**：本研究在大肠杆菌中成功表达了重组MAP2c蛋白(His标签)，通过体外微管共沉淀实验证实其能够显著增强微管稳定性。研究还发现MAP2c的N端结构域对微管结合至关重要，为神经元发育中微管动力学调控提供了分子机制依据。
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2. **文献名称**：*"Phosphorylation-Dependent Regulation of MAP2 Recombinant Protein in Axon Growth"*
**作者**：Suzuki, A. et al. (2012)
**摘要**：作者利用昆虫细胞系统表达全长重组MAP2蛋白，探究其磷酸化修饰对功能的影响。实验表明，CDK5激酶介导的MAP2磷酸化会抑制其与微管结合的能力，进而影响神经元轴突的延伸，揭示了MAP2在神经可塑性中的调控途径。
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3. **文献名称**：*"Structural Characterization of MAP2 Recombinant Protein Using Cryo-EM"*
**作者**：Gupta, N. & Lee, K. (2020)
**摘要**：该研究通过冷冻电镜技术解析了重组MAP2蛋白(表达于哺乳动物细胞)的N端微管结合域高分辨率结构，揭示了其与α/β微管蛋白异二聚体的结合模式。结果为开发靶向MAP2的神经退行性疾病治疗策略提供了结构基础。
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注：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar检索真实发表的论文。
**Background of MAP2 Recombinant Protein**
Microtubule-associated protein 2 (MAP2) is a critical cytoskeletal protein predominantly expressed in neurons, where it plays essential roles in stabilizing microtubules (MTs), regulating neuronal morphology, and supporting synaptic plasticity. Belonging to the MAP family, MAP2 exists in multiple isoforms generated through alternative splicing, with high-molecular-weight isoforms (e.g., MAP2a/b) localized to dendrites and smaller isoforms (e.g., MAP2c) present during early development. Its structure includes an N-terminal projection domain involved in interactions with other cytoskeletal components and signaling molecules, and a C-terminal MT-binding domain that stabilizes MT networks by promoting polymerization and reducing disassembly.
MAP2 is crucial for neurite outgrowth, dendritic arborization, and maintaining neuronal polarity. Dysregulation of MAP2 has been linked to neurodegenerative diseases (e.g., Alzheimer’s) and neurodevelopmental disorders. To study its functions, recombinant MAP2 proteins are produced using expression systems like *E. coli* or mammalian cells. These recombinant proteins retain key functional domains, enabling in vitro studies on MT dynamics, neuronal differentiation, and disease mechanisms.
Recombinant MAP2 is widely used in neuroscience research, including assays to screen MT-targeting drugs, model neurodegenerative pathologies, or engineer neuronal-like environments in cell cultures. Its availability also supports the development of diagnostic tools or therapeutic strategies targeting MT stability in neurological conditions. By providing a controlled and scalable source of functional MAP2. recombinant technology advances both basic and translational studies in neurobiology.
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