| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEF3 |
| Uniprot No | P07197 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 487-740aa |
| 氨基酸序列 | EKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKE |
| 预测分子量 | 34.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEF3(神经丝重链)重组蛋白研究的参考文献示例(注：以下内容为虚构示例，仅供格式参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：Expression and Purification of Recombinant NEF3 in Escherichia coli for Structural Analysis
**作者**：Smith A, et al.
**摘要**：本研究报道了通过大肠杆菌系统高效表达NEF3重组蛋白的优化方法，利用His标签纯化技术获得高纯度蛋白，并通过圆二色谱和电子显微镜验证其二级结构和聚合特性，为神经丝蛋白的体外研究奠定基础。
2. **文献名称**：Functional Characterization of NEF3 Recombinant Protein in Axonal Transport Assays
**作者**：Chen L, et al.
**摘要**：通过杆状病毒-昆虫细胞系统表达NEF3重组蛋白，并证明其在体外模型中调控微管稳定性和轴突运输的功能，揭示了NEF3异常聚集与神经退行性疾病的潜在关联。
3. **文献名称**：NEF3 Phosphorylation Mutants Generated by Recombinant Technology Reveal Role in Neurodegeneration
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物细胞表达体系构建NEF3磷酸化位点突变体重组蛋白，发现特定位点的磷酸化修饰显著影响神经丝网络的稳定性，为阿尔茨海默病中神经丝异常提供了机制解释。
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**注意**：以上文献信息为示例，实际研究中请通过**PubMed**、**Google Scholar**或**Web of Science**检索真实文献(关键词：NEF3 recombinant protein/Neurofilament Heavy expression)。
**Background of NEF3 Recombinant Protein**
NEF3. also known as neurofilament medium polypeptide (NF-M), is a critical structural component of neuronal intermediate filaments, primarily expressed in neurons. As part of the neurofilament triplet proteins (NEFH, NEFM, NEF3), it contributes to the cytoskeletal framework, maintaining axonal integrity, regulating diameter, and facilitating intracellular transport. Neurofilaments are biomarkers for neuronal health, with elevated levels in bodily fluids often indicating neurodegeneration or injury.
Recombinant NEF3 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper post-translational modifications. The purified protein retains functional epitopes, enabling its use in studying neurodegenerative mechanisms, such as Alzheimer’s disease, ALS, and traumatic brain injury. Researchers employ NEF3 recombinant proteins in immunoassays (ELISA, Western blot), antibody development, and *in vitro* models to explore axonal degeneration pathways.
Its role in disease pathogenesis and diagnostic potential has driven interest in NEF3 as a therapeutic target. By analyzing interactions with tau, amyloid-beta, or other neurotoxic aggregates, scientists aim to unravel its contribution to disease progression. Additionally, recombinant NEF3 supports standardization of diagnostic tools, improving accuracy in detecting neurofilament-related pathologies.
Overall, NEF3 recombinant protein serves as a vital tool for advancing neuroscience research, bridging gaps between molecular studies and clinical applications in neurology.
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