纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | NCDN |
Uniprot No | Q9UBB6 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-729aa |
氨基酸序列 | MSCCDLAAAGQLGKASIMASDCEPALNQAEGRNPTLERYLGALREAKNDS EQFAALLLVTKAVKAGDIDAKTRRRIFDAVGFTFPNRLLTTKEAPDGCPD HVLRALGVALLACFCSDPELAAHPQVLNKIPILSTFLTARGDPDDAARRS MIDDTYQCLTAVAGTPRGPRHLIAGGTVSALCQAYLGHGYGFDQALALLV GLLAAAETQCWKEAEPDLLAVLRGLSEDFQKAEDASKFELCQLLPLFLPP TTVPPECYRDLQAGLARILGSKLSSWQRNPALKLAARLAHACGSDWIPAG SSGSKFLALLVNLACVEVRLALEETGTEVKEDVVTACYALMELGIQECTR CEQSLLKEPQKVQLVSVMKEAIGAVIHYLLQVGSEKQKEPFVFASVRILG AWLAEETSSLRKEVCQLLPFLVRYAKTLYEEAEEANDLSQQVANLAISPT TPGPTWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWEL TSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLM TSLPALVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAI LFLSQSHVARATPGSDQAVLALSPEYEGIWADLQELWFLGMQAFTGCVPL LPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRL CREAMRLQAGEETASHYRMAALEQCLSEP |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCDN重组蛋白的3篇参考文献示例(注:以下内容为模拟示例,实际文献需根据具体数据库检索):
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1. **文献名称**: "High-yield production of recombinant Neurochondrin in HEK293 cells and its role in neurite outgrowth"
**作者**: Tanaka, K. et al.
**摘要**: 研究利用HEK293哺乳动物表达系统高效表达了重组NCDN蛋白,并证明其通过激活ERK信号通路显著促进海马神经元的轴突生长。
2. **文献名称**: "Optimization of Neurochondrin (NCDN) expression in E. coli for structural studies"
**作者**: Müller, R. & Schmidt, A.
**摘要**: 报道了通过大肠杆菌表达系统生产NCDN重组蛋白的优化策略,采用融合标签提高可溶性表达,并利用镍柱亲和层析纯化,为后续结构功能研究奠定基础。
3. **文献名称**: "Recombinant NCDN interacts with 14-3-3 proteins: Implications for neurodevelopmental disorders"
**作者**: Chen, L. et al.
**摘要**: 通过体外pull-down实验证实重组NCDN蛋白与14-3-3家族蛋白特异性结合,提示其在神经发育异常疾病中的潜在调控机制。
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如需获取真实文献,建议在PubMed或Google Scholar中检索关键词“recombinant Neurochondrin”或“NCDN protein expression”。若研究领域较为小众,可扩展至相关功能研究(如神经发育、蛋白互作等)。
**Background of NCDN Recombinant Protein**
Neurochondrin (NCDN) is a cytoplasmic protein predominantly expressed in neuronal tissues, playing critical roles in neuronal development, synaptic plasticity, and intracellular signaling. Initially identified through its association with brain-specific functions, NCDN is implicated in regulating calcium signaling pathways, cytoskeletal dynamics, and cell survival mechanisms. Structurally, it contains conserved domains, including EF-hand motifs, which facilitate calcium-binding activities, and regions mediating interactions with signaling molecules like calmodulin.
The recombinant NCDN protein is engineered using genetic engineering techniques, where the *NCDN* gene is cloned into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to produce purified, functional protein. This approach allows researchers to study NCDN's biochemical properties, post-translational modifications, and interactions in controlled environments. Recombinant NCDN is essential for *in vitro* and *in vivo* studies, particularly in elucidating its role in neurological disorders. For instance, dysregulation of NCDN has been linked to neurodegenerative diseases, psychiatric conditions, and brain injury responses, highlighting its potential as a therapeutic target or biomarker.
Additionally, recombinant NCDN aids in antibody development for diagnostic tools and provides a standardized reagent for comparative studies across experimental models. Its application extends to drug discovery, where modulating NCDN-related pathways could offer strategies for treating cognitive impairments or neural degeneration. Despite progress, questions remain regarding its precise molecular mechanisms and tissue-specific functions, driving ongoing research to unravel its full physiological and pathological significance.
In summary, recombinant NCDN protein serves as a vital tool for advancing neuroscience research, bridging molecular insights with translational opportunities in neurology.
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