| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | A2BP1 |
| Uniprot No | Q9NWB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-397aa |
| 氨基酸序列 | MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP EYTGQTTVPE HTLNLYPPAQ THSEQSPADT SAQTVSGTAT QTDDAAPTDG QPQTQPSENT ENKSQPKRLH VSNIPFRFRD PDLRQMFGQF GKILDVEIIF NERGSKGFGF VTFENSADAD RAREKLHGTV VEGRKIEVNN ATARVMTNKK TVNPYTNGWK LNPVVGAVYS PEFYAGTVLL CQANQEGSSM YSAPSSLVYT SAMPGFPYPA ATAAAAYRGA HLRGRGRTVY NTFRAAAPPP PIPAYGGVVY QDGFYGADIY GGYAAYRYAQ PTPATAAAYS DSYGRVYAAD PYHHALAPAP TYGVGAMNAF APLTDAKTRS HADDVGLVLS SLQASIYRGG YNRFAPY |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于A2BP1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "Characterization of recombinant A2BP1 in neuronal RNA splicing regulation"
**作者**: Smith J, et al.
**摘要**: 研究通过重组A2BP1蛋白在体外模型中验证其作为RNA结合蛋白的功能，发现其通过调控mRNA剪接参与神经突触可塑性，可能与自闭症谱系障碍相关。
2. **文献名称**: "Expression and purification of human A2BP1 recombinant protein for structural studies"
**作者**: Lee S, Kim H.
**摘要**: 报道了利用大肠杆菌表达系统高效制备可溶性A2BP1重组蛋白的方法，并通过X射线晶体学初步解析其结构域构象，为功能研究提供基础。
3. **文献名称**: "A2BP1 recombinant protein modulates TDP-43 aggregation in ALS cellular models"
**作者**: Rodriguez CM, et al.
**摘要**: 发现重组A2BP1蛋白能够抑制TDP-43蛋白的异常聚集，提示其在肌萎缩侧索硬化症(ALS)中的潜在治疗作用，并探索了其分子互作机制。
注：以上文献信息为示例性概括，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"A2BP1 recombinant protein"或"ATXN2BP1 expression"查找最新研究。
A2BP1 (Ataxin-2 Binding Protein 1), also known as FOX1 or Rbfox1. is an RNA-binding protein encoded by the RBFOX1 gene. It belongs to the RBFOX family of splicing regulators, which play critical roles in post-transcriptional RNA processing, including alternative splicing, mRNA stability, and translation. A2BP1 is highly expressed in neuronal tissues, muscle, and heart, where it regulates the splicing of transcripts involved in synaptic transmission, ion channel function, and neuronal development. Its targets include genes associated with autism spectrum disorders, epilepsy, and other neurological conditions.
The recombinant A2BP1 protein is engineered for in vitro studies to investigate its molecular interactions and functional mechanisms. Typically produced in bacterial (e.g., E. coli) or mammalian expression systems, the recombinant protein retains RNA-binding activity through its conserved RNA recognition motif (RRM). Researchers utilize it to map RNA-protein interactions, study splicing regulation in cellular models, or explore its role in diseases. For instance, A2BP1 dysregulation has been implicated in neurodegeneration, with reduced levels observed in amyotrophic lateral sclerosis (ALS) and spinocerebellar ataxia type 2 (SCA2), where it interacts with mutant ataxin-2.
Purified recombinant A2BP1 often includes affinity tags (e.g., His-tag, GST) for isolation and may be used in pull-down assays, electrophoretic mobility shift assays (EMSAs), or structural studies. Its application extends to drug discovery efforts targeting RNA-protein networks in neurological disorders. Ongoing research aims to clarify how A2BP1-mediated splicing defects contribute to disease pathogenesis and whether modulating its activity could offer therapeutic potential.
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