| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYBPC1 |
| Uniprot No | Q00872 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1141aa |
| 氨基酸序列 | MPEPTKKEENEVPAPAPPPEEPSKEKEAGTTPAKDWTLVETPPGEEQAKQNANSQLSILFIEKPQGGTVKVGEDITFIAKVKAEDLLRKPTIKWFKGKWMDLASKAGKHLQLKETFERHSRVYTFEMQIIKAKDNFAGNYRCEVTYKDKFDSCSFDLEVHESTGTTPNIDIRSAFKRSGEGQEDAGELDFSGLLKRREVKQQEEEPQVDVWELLKNAKPSEYEKIAFQYGITDLRGMLKRLKRMRREEKKSAAFAKILDPAYQVDKGGRVRFVVELADPKLEVKWYKNGQEIRPSTKYIFEHKGCQRILFINNCQMTDDSEYYVTAGDEKCSTELFVREPPIMVTKQLEDTTAYCGERVELECEVSEDDANVKWFKNGEEIIPGPKSRYRIRVEGKKHILIIEGATKADAAEYSVMTTGGQSSAKLSVDLKPLKILTPLTDQTVNLGKEICLKCEISENIPGKWTKNGLPVQESDRLKVVHKGRIHKLVIANALTEDEGDYVFAPDAYNVTLPAKVHVIDPPKIILDGLDADNTVTVIAGNKLRLEIPISGEPPPKAMWSRGDKAIMEGSGRIRTESYPDSSTLVIDIAERDDSGVYHINLKNEAGEAHASIKVKVVDFPDPPVAPTVTEVGDDWCIMNWEPPAYDGGSPILGYFIERKKKQSSRWMRLNFDLCKETTFEPKKMIEGVAYEVRIFAVNAIGISKPSMPSRPFVPLAVTSPPTLLTVDSVTDTTVTMRWRPPDHIGAAGLDGYVLEYCFEGSTSAKQSDENGEAAYDLPAEDWIVANKDLIDKTKFTITGLPTDAKIFVRVKAVNAAGASEPKYYSQPILVKEIIEPPKIRIPRHLKQTYIRRVGEAVNLVIPFQGKPRPELTWKKDGAEIDKNQINIRNSETDTIIFIRKAERSHSGKYDLQVKVDKFVETASIDIQIIDRPGPPQIVKIEDVWGENVALTWTPPKDDGNAAITGYTIQKADKKSMEWFTVIEHYHRTSATITELVIGNEYYFRVFSENMCGLSEDATMTKESAVIARDGKIYKNPVYEDFDFSEAPMFTQPLVNTYAIAGYNATLNCSVRGNPKPKITWMKNKVAIVDDPRYRMFSNQGVCTLEIRKPSPYDGGTYCCKAVNDLGTVEIECKLEVKVIAQ |
| 预测分子量 | 128 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYBPC1重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**: "Expression and purification of human MYBPC1 recombinant protein for structural studies"
**作者**: Smith J, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统成功制备了人源MYBPC1重组蛋白，优化了纯化条件，并通过圆二色光谱验证其二级结构完整性，为后续功能研究奠定基础。
2. **文献名称**: "Functional characterization of MYBPC1 mutations associated with skeletal muscle disease"
**作者**: Lee S, et al.
**摘要**: 利用重组MYBPC1蛋白进行体外结合实验，发现特定突变体与肌球蛋白的结合能力显著降低，揭示了MYBPC1基因突变导致肌肉功能障碍的分子机制。
3. **文献名称**: "Proteolytic processing of MYBPC1 regulates its interaction with myosin filaments"
**作者**: Zhang R, et al.
**摘要**: 研究通过重组蛋白技术证明，钙蛋白酶介导的MYBPC1剪切可调节其与肌丝的结合能力，提示该蛋白在肌肉动态收缩调控中的关键作用。
备注：以上文献信息为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。若需具体文献，建议结合最新研究补充。
**Background of MYBPC1 Recombinant Protein**
MYBPC1 (Myosin Binding Protein C1) is a skeletal muscle-specific isoform belonging to the Myosin Binding Protein C (MYBPC) family, which plays a critical role in regulating sarcomere structure and function. Expressed predominantly in fast-twitch muscle fibers, MYBPC1 binds to myosin heavy chains and titin within the A-band of sarcomeres, contributing to the assembly, stability, and modulation of muscle contraction. It acts as a structural and regulatory component, influencing cross-bridge cycling kinetics and calcium sensitivity during force generation. Mutations in the *MYBPC1* gene have been linked to distal arthrogryposis and congenital myopathies, highlighting its importance in muscle development and function.
Recombinant MYBPC1 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, biologically active protein for *in vitro* studies. This tool enables researchers to investigate MYBPC1’s interactions with sarcomeric proteins, its role in mechanotransduction, and its involvement in disease mechanisms. Studies leveraging recombinant MYBPC1 have advanced our understanding of its phosphorylation-dependent regulatory functions and potential therapeutic targets for neuromuscular disorders. Additionally, it serves as an antigen for antibody development, aiding diagnostic and functional assays.
Overall, MYBPC1 recombinant protein is vital for dissecting sarcomere biology and exploring therapeutic strategies for muscle-related diseases.
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