| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSFA2 |
| Uniprot No | P28290 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1259aa |
| 氨基酸序列 | MDRPLSSSAEAEEELEWQVASRRRKAWAKCRSSWQASETEDLSTEATTQDEEEDEEEDLPGAQLPAAGGRGNVPNEKIAIWLKDCRTPLGASLDEQSSSTLKGVLVRNGGSFEDDLSLGAEANHLHESDAQIENCNNILAKERRLQFHQKGRSMNSTGSGKSSGTVSSVSELLELYEEDPEEILYNLGFGRDEPDIASKIPSRFFNSSSFAKGIDIKVFLSAQMQRMEVENPNYALTSRFRQIEVLTTVANAFSSLYSQVSGTPLQRIGSMSSVTSNKETDPPPPLTRSNTANRLMKTLSKLNLCVDKTEKGESSSPSPSAEKGKILNVSVIEESGNKNDQKSQKIMKKKESSSMLATVKEEVSGSSAAVTENADSDRISDEANSNFNQGTENEQSKETQSHESKLGEESGIVESKLDSDFNISSHSELENSSELKSVHISTPEKEPCAPLTIPSIRNIMTQQKDSFEMEEVQSTEGEAPHVPATYQLGLTKSKRDHLLRTASQHSDSSGFAEDSTDCLSLNHLQVQESLQAMGSSADSCDSETTVTSLGEDLATPTAQDQPYFNESEEESLVPLQKGLEKAAAVADKRKSGSQDFPQCNTIENTGTKQSTCSPGDHIIEITEVEEDLFPAETVELLREASAESDVGKSSESEFTQYTTHHILKSLASIEAKCSDMSSENTTGPPSSMDRVNTALQRAQMKVCSLSNQRMGRSLLKSKDLLKQRYLFAKAGYPLRRSQSLPTTLLSPVRVVSSVNVRLSPGKETRCSPPSFTYKYTPEEEQELEKRVMEHDGQSLVKSTIFISPSSVKKEEAPQSEAPRVEECHHGRTPTCSRLAPPPMSQSTCSLHSIHSEWQERPLCEHTRTLSTHSVPNISGATCSAFASPFGCPYSHRHATYPYRVCSVNPPSAIEMQLRRVLHDIRNSLQNLSQYPMMRGPDPAAAPYSTQKSSVLPLYENTFQELQVMRRSLNLFRTQMMDLELAMLRQQTMVYHHMTEEERFEVDQLQGLRNSVRMELQDLELQLEERLLGLEEQLRAVRMPSPFRSSALMGMCGSRSADNLSCPSPLNVMEPVTELMQEQSYLKSELGLGLGEMGFEIPPGESSESVFSQATSESSSVCSGPSHANRRTGVPSTASVGKSKTPLVARKKVFRASVALTPTAPSRTGSVQTPPDLESSEEVDAAEGAPEVVGPKSEVEEGHGKLPSMPAAEEMHKNVEQDELQQVIREIKESIVGEIRREIVSGLLAAVSSSKASNSKQDYH |
| 预测分子量 | 138,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SSFA2重组蛋白的3篇参考文献示例(注：文献信息为虚构，仅用于示例格式)：
1. **标题**：*Expression and Functional Characterization of Recombinant SSFA2 Protein in Sperm-Oocyte Interaction*
**作者**：Zhang L, et al.
**期刊**：*J Reprod Immunol* (2021)
**摘要**：本研究成功在大肠杆菌中表达并纯化了SSFA2重组蛋白，证实其在体外精子-卵子结合实验中增强粘附能力，提示SSFA2可能作为生殖医学中潜在的靶点。
2. **标题**：*Structural Analysis of SSFA2 Recombinant Protein and Its Role in Immune Regulation*
**作者**：Tanaka K, et al.
**期刊**：*Protein Sci* (2019)
**摘要**：通过X射线晶体学解析SSFA2重组蛋白的三维结构，发现其与免疫细胞表面受体CD36存在特异性结合，可能参与炎症反应的调控机制。
3. **标题**：*SSFA2 Recombinant Protein as a Diagnostic Marker for Male Infertility*
**作者**：Wang Y, et al.
**期刊**：*Andrology* (2020)
**摘要**：临床研究表明，SSFA2重组蛋白在少精症患者精液中表达显著降低，提示其可作为男性不育的新型生物标志物。
(注：实际文献需通过PubMed/Google Scholar等平台以“SSFA2 recombinant protein”为关键词检索获取。)
**Background of SSFA2 Recombinant Protein**
SSFA2 (structure-specific nuclease subunit A2), also known as ERCC5 or XPG, is a multifunctional protein encoded by the *ERCC5* gene in humans. It plays a critical role in DNA repair mechanisms, particularly nucleotide excision repair (NER), which addresses bulky DNA lesions caused by UV radiation or chemical agents. SSFA2 functions as an endonuclease, cleaving damaged DNA strands at specific sites during the repair process. Beyond NER, it participates in transcription-coupled repair (TCR) and interacts with other repair proteins like TFIIH and XPA, ensuring genomic stability.
The recombinant SSFA2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. Its recombinant version retains enzymatic activity, enabling studies on DNA repair pathways, cancer biology, and aging. Mutations in *ERCC5* are linked to xeroderma pigmentosum (XP), Cockayne syndrome, and increased cancer susceptibility, making SSFA2 a focal point for understanding genetic disorders and therapeutic development.
In biomedical research, recombinant SSFA2 is utilized to investigate DNA damage responses, screen for DNA repair-targeting drugs, and model disease mechanisms. Its role in maintaining genomic integrity also highlights its potential as a biomarker for cancer prognosis or as a target for precision therapies. Overall, SSFA2 recombinant protein serves as a vital tool for dissecting DNA repair dynamics and advancing translational applications.
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