| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LGALS3BP |
| Uniprot No | Q08380 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585aa |
| 氨基酸序列 | MTPPRLFWVWLLVAGTQGVNDGDMRLADGGATNQGRVEIFYRGQWGTVCD NLWDLTDASVVCRALGFENATQALGRAAFGQGSGPIMLDEVQCTGTEASL ADCKSLGWLKSNCRHERDAGVVCTNETRSTHTLDLSRELSEALGQIFDSQ RGCDLSISVNVQGEDALGFCGHTVILTANLEAQALWKEPGSNVTMSVDAE CVPMVRDLLRYFYSRRIDITLSSVKCFHKLASAYGARQLQGYCASLFAIL LPQDPSFQMPLDLYAYAVATGDALLEKLCLQFLAWNFEALTQAEAWPSVP TDLLQLLLPRSDLAVPSELALLKAVDTWSWGERASHEEVEGLVEKIRFPM MLPEELFELQFNLSLYWSHEALFQKKTLQALEFHTVPFQLLARYKGLNLT EDTYKPRIYTSPTWSAFVTDSSWSARKSQLVYQSRRGPLVKYSSDYFQAP SDYRYYPYQSFQTPQHPSFLFQDKRVSWSLVYLPTIQSCWNYGFSCSSDE LPVLGLTKSGGSDRTIAYENKALMLCEGLFVADVTDFEGWKAATPSALDT NSSKSTSSFPCPAGHFNGFRTVIRPFYLTNSSGVD |
| 预测分子量 | 90 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LGALS3BP重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*LGALS3BP regulates antiviral responses through interaction with galectin-3 in human cells*
**作者**：Chen Y. et al.
**摘要**：该研究利用重组LGALS3BP蛋白探究其与galectin-3的相互作用机制，发现其通过激活干扰素信号通路增强宿主对RNA病毒(如流感病毒)的先天免疫应答。
2. **文献名称**：*Recombinant LGALS3BP as a potential biomarker for early cancer detection*
**作者**：Smith J.R. et al.
**摘要**：文章报道了重组LGALS3BP蛋白在多种癌症患者血清中的高表达特性，验证其作为诊断标志物的潜力，并揭示其通过调控肿瘤微环境促进转移的可能机制。
3. **文献名称**：*Structural characterization of LGALS3BP and its role in extracellular matrix remodeling*
**作者**：Tanaka K. et al.
**摘要**：通过表达纯化重组LGALS3BP蛋白，解析其结构域功能，证明其通过结合基质金属蛋白酶(MMPs)参与细胞外基质的动态平衡，为靶向治疗纤维化疾病提供依据。
(注：以上文献信息为示例，实际引用时需核实具体来源及发表年份。)
LGALS3BP (Galectin-3 Binding Protein), also known as 90K or MAC-2BP, is a secreted glycoprotein implicated in diverse physiological and pathological processes. It was initially identified as a ligand for galectin-3. a lectin involved in immune regulation, cell adhesion, and cancer progression. Structurally, LGALS3BP contains multiple functional domains, including a scavenger receptor cysteine-rich (SRCR) domain, a BTB/POZ domain, and several glycosylation sites, which contribute to its interactions with extracellular proteins and cell surface receptors.
This protein is expressed in various tissues and plays roles in modulating immune responses, extracellular matrix organization, and cell signaling. It acts as a multifunctional adaptor molecule, binding to galectin-3. integrins, collagens, and other immune-related molecules like CD4 and CD8. These interactions link LGALS3BP to processes such as viral entry inhibition, tumor suppression, and inflammation. Elevated levels of LGALS3BP are observed in cancers, autoimmune diseases, and viral infections (e.g., HIV, HCV), where it may serve as a biomarker for disease progression or therapeutic targeting.
Recombinant LGALS3BP is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications, particularly glycosylation critical for its functional activity. Purified recombinant forms are used in research to study its role in tumor microenvironments, immune evasion, and as a potential therapeutic agent or diagnostic tool. Current studies focus on its dual roles in cancer—acting as both a tumor suppressor by inhibiting angiogenesis and a promoter of metastasis through immune modulation—highlighting its context-dependent biological significance.
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