| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPR2 |
| Uniprot No | P20594 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-458aa |
| 氨基酸序列 | RNLTLAVVLPEHNLSYAWAWPRVGPAVALAVEALGRALPVDLRFVSSELEGACSEYLAPLSAVDLKLYHDPDLLLGPGCVYPAASVARFASHWRLPLLTAGAVASGFSAKNDHYRTLVRTGPSAPKLGEFVVTLHGHFNWTARAALLYLDARTDDRPHYFTIEGVFEALQGSNLSVQHQVYAREPGGPEQATHFIRANGRIVYICGPLEMLHEILLQAQRENLTNGDYVFFYLDVFGESLRAGPTRATGRPWQDNRTREQAQALREAFQTVLVITYREPPNPEYQEFQNRLLIRAREDFGVELGPSLMNLIAGCFYDGILLYAEVLNETIQEGGTREDGLRIVEKMQGRRYHGVTGLVVMDKNNDRETDFVLWAMGDLDSGDFQPAAHYSGAEKQIWWTGRPIPWVKGAPPSDNPPCAFDLDDPSCDKTPLSTLAI |
| 预测分子量 | 50.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPR2重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Structural characterization of the extracellular domain of NPR2*
**作者**：Suga S, et al.
**摘要**：该研究解析了NPR2重组蛋白的胞外结构域三维结构，揭示了其与配体C型利钠肽(CNP)结合的分子机制，为理解受体激活和信号转导提供了结构基础。
2. **文献名称**：*Functional analysis of recombinant NPR2 in ligand binding*
**作者**：Potter LR, Hunter T.
**摘要**：通过重组表达NPR2的胞外段，验证了其对CNP的高亲和力结合特性，并发现其与细胞膜锚定形式的功能差异，提示重组蛋白在体外研究中的潜在应用价值。
3. **文献名称**：*NPR2 mutations impair cGMP production in recombinant expression systems*
**作者**：Hirschhaeuser C, et al.
**摘要**：利用哺乳动物细胞重组表达系统，发现骨发育异常相关NPR2突变体导致受体鸟苷酸环化酶活性显著下降，证明重组蛋白技术可用于疾病致病机制的功能验证。
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**备注**：以上文献信息为示例性内容，实际研究中建议通过PubMed或Web of Science以“NPR2 recombinant protein”为关键词检索最新论文，重点关注重组表达方法、结构功能研究或疾病模型应用方向。
**Background of NPR2 Recombinant Protein**
Natriuretic peptide receptor 2 (NPR2), also known as guanylate cyclase-B (GC-B), is a transmembrane receptor critical for mediating cellular responses to C-type natriuretic peptide (CNP), a member of the natriuretic peptide family. NPR2 functions as a homodimer, binding CNP to activate its intracellular guanylate cyclase domain, catalyzing the conversion of GTP to cyclic guanosine monophosphate (cGMP). This cGMP-dependent signaling pathway regulates diverse physiological processes, including skeletal growth, cardiovascular homeostasis, and reproductive functions. Notably, NPR2 is indispensable for endochondral ossification, as mutations in *NPR2* are linked to skeletal dysplasia disorders such as acromesomelic dysplasia Maroteaux type.
Recombinant NPR2 protein is engineered using biotechnological platforms (e.g., bacterial, insect, or mammalian expression systems) to produce purified, functional receptor variants for research and therapeutic applications. Its production enables structural studies, ligand-receptor interaction analyses, and high-throughput drug screening. Recombinant NPR2 is particularly valuable in elucidating mechanisms underlying CNP/NPR2 signaling disruptions in diseases, including short stature syndromes and cardiovascular disorders.
In recent years, NPR2 has gained attention as a therapeutic target. For example, activating NPR2 signaling via CNP analogs (e.g., vosoritide) has shown promise in treating achondroplasia. Conversely, NPR2 dysfunction in cancers highlights its context-dependent roles in cell proliferation and apoptosis. Recombinant NPR2 also aids in developing diagnostic tools, such as assays to measure CNP levels or detect autoantibodies in autoimmune conditions.
Overall, NPR2 recombinant protein serves as a vital tool for advancing understanding of cGMP-mediated pathways and bridging translational research from molecular mechanisms to clinical interventions.
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