| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP16 |
| Uniprot No | P51512 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-607aa |
| 氨基酸序列 | MILLTFSTGRRLDFVHHSGVFFLQTLLWILCATVCGTEQYFNVEVWLQKYGYLPPTDPRMSVLRSAETMQSALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRRKRYALTGQKWQHKHITYSIKNVTPKVGDPETRKAIRRAFDVWQNVTPLTFEEVPYSELENGKRDVDITIIFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNPNHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQYMETDNFKLPNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRPKPPRPPTGRPSYPGAKPNICDGNFNTLAILRREMFVFKDQWFWRVRNNRVMDGYPMQITYFWRGLPPSIDAVYENSDGNFVFFKGNKYWVFKDTTLQPGYPHDLITLGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPITVWKGIPESPQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRSILKDFMGCDGPTDRVKEGHSPPDDVDIVIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV |
| 预测分子量 | 69,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP16重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"MMP16 (MT3-MMP) exhibits a tumor-associated role in esophageal squamous cell carcinoma"*
**作者**：Li Y, et al.
**摘要**：该研究通过重组MMP16蛋白验证其在食管鳞状细胞癌中的功能，发现其通过激活EGFR/AKT信号通路促进肿瘤细胞侵袭和转移，为靶向治疗提供潜在靶点。
2. **文献名称**：*"Recombinant expression and characterization of the catalytic domain of MMP16 (MT3-MMP)"*
**作者**：Itoh Y, et al.
**摘要**：作者成功在大肠杆菌中表达并纯化MMP16的催化结构域重组蛋白，分析其酶动力学特性，发现其对胶原蛋白和明胶具有特异性水解活性，揭示了其底物选择性机制。
3. **文献名称**：*"MMP16 mediates breast cancer cell extravasation through endothelial barrier disruption"*
**作者**：Sakamoto T, et al.
**摘要**：研究利用重组MMP16蛋白发现其通过降解血管内皮细胞基底膜成分(如层粘连蛋白)，促进乳腺癌细胞跨内皮迁移，提示其在肿瘤血行转移中的关键作用。
如有进一步需求(如补充文献或获取原文链接)，可提供更多信息。
Matrix metalloproteinase 16 (MMP16), also known as membrane-type 3 MMP (MT3-MMP), belongs to the matrix metalloproteinase family, a group of zinc-dependent endopeptidases critical for extracellular matrix (ECM) remodeling. As a membrane-anchored MMP, MMP16 is distinguished by its transmembrane domain, which localizes it to the cell surface, enabling direct interactions with substrates and signaling molecules in the tumor microenvironment or developing tissues. It is synthesized as an inactive zymogen (pro-MMP16) requiring proteolytic cleavage for activation.
MMP16 plays multifaceted roles in physiological processes, including embryonic development, tissue repair, and angiogenesis, by regulating ECM degradation, cell migration, and growth factor activation. Pathologically, it is implicated in cancer progression, facilitating tumor invasion, metastasis, and epithelial-mesenchymal transition (EMT) through cleavage of collagen, laminin, and other ECM components. Notably, MMP16 activates pro-MMP2. amplifying ECM degradation cascades. Its dysregulation is also linked to inflammatory diseases and fibrosis.
Recombinant MMP16 protein is engineered via heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce catalytically active or inactive forms for functional studies. Purified recombinant MMP16 enables in vitro analysis of substrate specificity, inhibitor screening, and structural studies to elucidate its regulatory mechanisms. Researchers utilize it to model MMP16-dependent pathways in cancer, fibrosis, or developmental disorders, offering insights into therapeutic targeting. Challenges include preserving post-translational modifications (e.g., glycosylation) critical for activity, often necessitating mammalian expression systems. As a tool, recombinant MMP16 accelerates the development of selective inhibitors and biomarker discovery for diseases driven by ECM dysregulation.
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