| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SDF4 |
| Uniprot No | Q9BRK5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-362aa |
| 氨基酸序列 | RPANHSSTRERVANREENEILPPDHLNGVKLEMDGHLNRGFHQEVFLGKDLGGFDEDAEPRRSRRKLMVIFSKVDVNTDRKISAKEMQRWIMEKTAEHFQEAMEESKTHFRAVDPDGDGHVSWDEYKVKFLASKGHSEKEVADAIRLNEELKVDEETQEVLENLKDRWYQADSPPADLLLTEEEFLSFLHPEHSRGMLRFMVKEIVRDLDQDGDKQLSVPEFISLPVGTVENQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTAEELESYMDPMNEYNALNEAKQMIAVADENQNHHLEPEEVLKYSEFFTGSKLVDYARSVHEEF |
| 预测分子量 | 45.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SDF4(Stromal Cell-Derived Factor 4)重组蛋白的参考文献示例(注：SDF4相关研究较少，以下为模拟示例，实际文献需根据具体研究方向检索)：
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1. **文献名称**：*Expression and functional characterization of recombinant SDF4 in regulating calcium-dependent exocytosis*
**作者**：Smith A, et al.
**摘要**：研究报道了在大肠杆菌系统中高效表达重组SDF4蛋白的方法，并验证其通过钙离子结合调控细胞分泌过程的功能，揭示了其在神经内分泌细胞中的作用。
2. **文献名称**：*Structural insights into SDF4-Ca²⁺ interaction and implications for secretory pathway modulation*
**作者**：Li Y, et al.
**摘要**：通过X射线晶体学解析SDF4的钙结合结构域，发现其与内质网钙稳态的关联，重组SDF4蛋白可调节细胞内囊泡运输效率。
3. **文献名称**：*SDF4 knockout mice exhibit impaired protein secretion and endoplasmic reticulum stress*
**作者**：Tanaka K, et al.
**摘要**：利用基因编辑技术构建SDF4敲除小鼠模型，发现重组SDF4蛋白回补可缓解内质网应激，表明其在维持分泌通路中的关键作用。
4. **文献名称**：*Recombinant SDF4 as a potential therapeutic agent for secretory pathway disorders*
**作者**：Wang H, et al.
**摘要**：体外实验表明，重组SDF4蛋白能够增强细胞对病理条件下分泌压力的耐受性，为治疗相关疾病提供了分子基础。
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**注意**：SDF4(属于分泌型钙结合蛋白家族)的研究相对较少，实际文献可能需要结合具体物种(如人、小鼠)或疾病背景检索。建议通过PubMed或Web of Science以关键词“SDF4 recombinant”“SDF4 secretion”进一步筛选。
**Background of SDF4 Recombinant Protein**
Stromal cell-derived factor 4 (SDF4), also known as stromal cell-derived receptor 4 or CABIN1. is a calcium-binding protein belonging to the calsequestrin family. It is primarily localized in the endoplasmic reticulum (ER), where it plays a critical role in regulating intracellular calcium storage and homeostasis. SDF4 interacts with ER calcium pumps and channels, facilitating calcium buffering and signaling, which are essential for cellular processes such as proliferation, migration, and apoptosis.
The gene encoding SDF4 is located on chromosome 1p36.13 in humans and is evolutionarily conserved, reflecting its fundamental role in cellular physiology. Structurally, SDF4 contains multiple low-affinity, high-capacity calcium-binding sites, enabling it to sequester large amounts of calcium within the ER lumen. This function is vital for maintaining calcium-dependent signaling pathways, including those mediated by inositol trisphosphate (IP3) and ryanodine receptors.
Recombinant SDF4 is produced using biotechnological platforms, such as bacterial or mammalian expression systems, to ensure proper folding and post-translational modifications. Its production enables researchers to study calcium regulation mechanisms, ER stress responses, and diseases linked to calcium dysregulation, including neurodegenerative disorders, cardiovascular diseases, and cancer. Notably, SDF4 has been implicated in tumor progression, as altered calcium signaling can promote cancer cell survival and metastasis.
Beyond basic research, recombinant SDF4 holds potential therapeutic applications. It may serve as a tool to modulate calcium signaling in pathological conditions or act as a biomarker for ER stress-related diseases. Ongoing studies aim to elucidate its interactions with other ER proteins and its role in disease mechanisms, highlighting its significance in both cell biology and translational medicine.
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