| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FPR2 |
| Uniprot No | P25090 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351aa |
| 氨基酸序列 | METNFSTPLNEYEEVSYESAGYTVLRILPLVVLGVTFVLGVLGNGLVIWVAGFRMTRTVTTICYLNLALADFSFTATLPFLIVSMAMGEKWPFGWFLCKLIHIVVDINLFGSVFLIGFIALDRCICVLHPVWAQNHRTVSLAMKVIVGPWILALVLTLPVFLFLTTVTIPNGDTYCTFNFASWGGTPEERLKVAITMLTARGIIRFVIGFSLPMSIVAICYGLIAAKIHKKGMIKSSRPLRVLTAVVASFFICWFPFQLVALLGTVWLKEMLFYGKYKIIDILVNPTSSLAFFNSCLNPMLYVFVGQDFRERLIHSLPTSLERALSEDSAPTNDTAANSASPPAETELQAM |
| 预测分子量 | 40.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FPR2重组蛋白的代表性文献概览：
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1. **文献名称**: "Formyl peptide receptor 2: Characterization of ligand-specific signaling and antagonist discovery"
**作者**: Chen X, et al.
**摘要**: 研究通过重组表达人源FPR2蛋白，分析其配体结合特性及信号转导机制，发现新型拮抗剂可抑制FPR2介导的中性粒细胞趋化作用。
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2. **文献名称**: "Structural basis of FPR2 activation by amyloidogenic peptides"
**作者**: Li Y, et al.
**摘要**: 利用重组FPR2蛋白的冷冻电镜结构解析，揭示了β淀粉样蛋白等病理多肽激活FPR2的分子机制，为神经炎症疾病治疗提供靶点依据。
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3. **文献名称**: "Recombinant FPR2 extracellular domain inhibits neutrophil infiltration in murine sepsis model"
**作者**: Gupta S, et al.
**摘要**: 通过大肠杆菌表达重组FPR2胞外结构域蛋白，证明其可通过竞争性结合炎症因子减轻脓毒症模型中的中性粒细胞过度浸润。
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4. **文献名称**: "Bioprocess optimization for high-yield FPR2 production in mammalian cells"
**作者**: Müller CE, et al.
**摘要**: 开发HEK293细胞中重组FPR2的高效表达纯化工艺，获得具有天然构象的功能性蛋白，适用于药物筛选和受体功能研究。
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提示：文献检索建议结合PubMed或SciFinder数据库，以“FPR2 recombinant”、“FPR2 expression”为关键词筛选近五年研究，重点关注信号机制、疾病模型应用及结构生物学方向。
**Background of FPR2 Recombinant Protein**
Formyl peptide receptor 2 (FPR2), also known as ALX/FPR2 or FPRL1. is a G protein-coupled receptor (GPCR) that plays a critical role in immune regulation and inflammatory responses. It belongs to the formyl peptide receptor family, which includes FPR1 and FPR3. FPR2 is characterized by its ability to bind a diverse range of ligands, including formylated peptides (e.g., bacterial or mitochondrial-derived peptides), lipid mediators (e.g., lipoxin A4. resolvin D1), and endogenous proteins (e.g., amyloid-beta, Annexin A1). This broad ligand specificity allows FPR2 to mediate both pro-inflammatory and anti-inflammatory signals, depending on the cellular context and ligand interaction.
FPR2 is predominantly expressed in immune cells, such as neutrophils, monocytes, and macrophages, where it regulates chemotaxis, phagocytosis, and cytokine release. It is also involved in resolving inflammation by promoting the clearance of apoptotic cells and enhancing the production of pro-resolving lipid mediators. Dysregulation of FPR2 has been linked to chronic inflammatory diseases (e.g., atherosclerosis, rheumatoid arthritis), neurodegenerative disorders (e.g., Alzheimer’s disease), and cancer progression.
Recombinant FPR2 protein is engineered through heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its structure, ligand interactions, and signaling mechanisms. This protein is essential for *in vitro* assays, drug screening, and antibody development. Researchers leverage recombinant FPR2 to explore its dual role in inflammation and tissue repair, aiming to design therapeutic agents that modulate its activity—either as agonists to enhance resolution or antagonists to suppress excessive inflammation. Its potential as a drug target continues to drive interest in immunology and pharmacology.
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