| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCC3 |
| Uniprot No | Q9UHW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1150aa |
| 氨基酸序列 | MHPPETTTKMASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS |
| 预测分子量 | 127 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCC3重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Molecular Cloning and Functional Characterization of KCC3. a Novel K-Cl Cotransporter*
**作者**：Race, J.E. et al.
**摘要**：该研究首次克隆了KCC3基因，并在HEK293细胞中重组表达，验证其作为钾-氯共转运蛋白的功能。实验表明KCC3在细胞体积调节和离子稳态中起关键作用，并发现其与遗传性神经疾病(如周围神经病变)的可能关联。
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2. **文献名称**：*Characterization of Phosphorylation Sites in the K-Cl Cotransporter KCC3*
**作者**：de Los Heros, P. et al.
**摘要**：通过重组表达KCC3蛋白，研究其磷酸化修饰位点及其对转运活性的调控。发现激酶WNK1通过磷酸化KCC3的N端结构域抑制其功能，揭示了细胞信号通路对离子转运的调控机制。
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3. **文献名称**：*Dysregulation of KCC3 in a Mouse Model of Hereditary Motor and Sensory Neuropathy*
**作者**：Howard, H.C. et al.
**摘要**：利用重组KCC3蛋白构建转基因小鼠模型，研究KCC3功能缺失如何导致遗传性运动和感觉神经病变(HMSN)。结果表明KCC3异常影响神经元离子平衡，进而引发轴突变性和运动障碍。
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**备注**：以上文献为示例性概括，实际文献需通过学术数据库(如PubMed、Google Scholar)检索确认。若需具体DOI或发表年份，可进一步提供关键词协助查找。
**Background of KCC3 Recombinant Protein**
The K⁺-Cl⁻ cotransporter 3 (KCC3), encoded by the *SLC12A6* gene, is a member of the SLC12A family of electroneutral cation-chloride cotransporters. It plays a critical role in maintaining cellular ion homeostasis, particularly in regulating intracellular Cl⁻ concentration and cell volume. KCC3 is widely expressed in the nervous system, kidneys, and other tissues, where it facilitates the coupled transport of potassium and chloride ions out of cells, often in response to osmotic stress or changes in cell volume.
Dysfunction of KCC3 is linked to human diseases. Loss-of-function mutations in *SLC12A6* cause Andermann syndrome, a rare autosomal recessive disorder characterized by peripheral neuropathy, agenesis of the corpus callosum, and developmental delays. Studies in animal models have further highlighted its importance in neuronal excitability, blood pressure regulation, and renal function.
Recombinant KCC3 protein is engineered for *in vitro* research to study its biochemical properties, regulatory mechanisms, and interactions with drugs or signaling molecules. It is typically produced using heterologous expression systems (e.g., mammalian HEK293 cells or insect cell lines) to ensure proper post-translational modifications and functional activity. Purified KCC3 recombinant protein serves as a tool for structural analysis (e.g., cryo-EM studies), inhibitor screening, and functional assays measuring ion transport kinetics.
Research leveraging KCC3 recombinant protein has advanced understanding of its role in pathologies such as epilepsy, hypertension, and neurodevelopmental disorders, while also aiding the development of targeted therapies. Its study remains pivotal in unraveling the complex interplay between ion transport and disease.
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