| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT23 |
| Uniprot No | Q9C075 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-422aa |
| 氨基酸序列 | MNSGHSFSQTPSASFHGAGGGWGRPRSFPRAPTVHGGAGGARISLSFTTR SCPPPGGSWGSGRSSPLLGGNGKATMQNLNDRLASYVEKVRALEEANMKL ESRILKWHQQRDPGSKKDYSQYEENITHLQEQIVDGKMTNAQIILLIDNA RMAVDDFNLKYENEHSFKKDLEIEVEGLRRTLDNLTIVTTDLEQEVEGMR KELILMKKHHEQEMEKHHVPSDFNVNVKVDTGPREDLIKVLEDMRQEYEL IIKKKHRDLDTWYKEQSAAMSQEAASPATVQSRQGDIHELKRTFQALEID LQTQYSTKSALENMLSETQSRYSCKLQDMQEIISHYEEELTQLRHELERQ NNEYQVLLGIKTHLEKEITTYRRLLEGESEGTREESKSSMKVFATPKIKA ITQETINGRLVLCQVNEIQKHA |
| 预测分子量 | 48,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT23重组蛋白的3篇参考文献示例(注：文献为模拟概括，实际需根据具体研究补充)：
1. **标题**：*KRT23 as a Biomarker for Colorectal Cancer Progression*
**作者**：Ismail TM, et al.
**摘要**：研究报道KRT23在结肠癌组织中高表达，并通过重组蛋白实验证实其与肿瘤侵袭性相关，可能作为预后标志物。
2. **标题**：*Recombinant KRT23 Expression and Purification in E. coli*
**作者**：Zhang Y, et al.
**摘要**：描述利用大肠杆菌系统高效表达并纯化重组KRT23蛋白的方法，为后续功能研究提供技术基础。
3. **标题**：*KRT23 Promotes Liver Fibrosis via TGF-β Pathway*
**作者**：Suzuki K, et al.
**摘要**：通过体外重组KRT23蛋白实验，揭示其通过激活TGF-β信号通路加剧肝纤维化过程的分子机制。
(提示：实际文献需通过PubMed、Google Scholar等平台以“KRT23 recombinant protein”为关键词检索获取。)
**Background of KRT23 Recombinant Protein**
KRT23 (Keratin 23) is a member of the keratin family, which comprises intermediate filament proteins essential for maintaining cellular structural integrity, particularly in epithelial tissues. As a type I acidic keratin, KRT23 is encoded by the *KRT23* gene and is predominantly expressed in differentiating epithelial cells under normal physiological conditions. However, its dysregulation has been implicated in various pathological processes, including cancer progression, inflammation, and tissue repair.
Recombinant KRT23 protein is produced using genetic engineering techniques, often in expression systems like *E. coli* or mammalian cells, to ensure high purity and bioactivity. This engineered protein retains the functional domains of native KRT23. enabling its use in studying molecular interactions, signaling pathways, and cellular mechanisms *in vitro*. Research has highlighted KRT23's role in cancer, where its overexpression correlates with aggressive phenotypes in colorectal, hepatocellular, and breast cancers. It promotes tumor cell proliferation, migration, and resistance to apoptosis, partly through interactions with pathways like Wnt/β-catenin and EMT (epithelial-mesenchymal transition).
Beyond cancer, KRT23 is explored as a potential diagnostic biomarker due to its elevated levels in certain diseases. Its recombinant form facilitates antibody development, drug screening, and functional assays, aiding the discovery of targeted therapies. Additionally, studies suggest KRT23 may influence inflammatory responses and tissue regeneration, broadening its relevance in biomedical research.
In summary, KRT23 recombinant protein serves as a critical tool for unraveling the protein's multifaceted roles in health and disease, offering translational potential for diagnostics and therapeutics.
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