| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNMD |
| Uniprot No | Q9H2S6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MAKNPPENCEDCHILNAEAFKSKKICKSLKICGLVFGILALTLIVLFWGSKHFWPEVPKKAYDMEHTFYSNGEKKKIYMEIDPVTRTEIFRSGNGTDETLEVHDFKNGYTGIYFVGLQKCFIKTQIKVIPEFSEPEEEIDENEEITTTFFEQSVIWVPAEKPIENRDFLKNSKILEICDNVTMYWINPTLISVSELQDFEEEGEDLHFPANEKKGIEQNEQWVVPQVKVEKTRHARQASEEELPINDYTENGIEFDPMLDERGYCCIYCRRGNRYCRRVCEPLLGYYPYPYCYQGGRVICRVIMPCNWWVARMLGRV |
| 预测分子量 | 37,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNMD(Tenomodulin)重组蛋白的3篇参考文献示例(注：内容为示例性概括，具体文献需根据实际检索结果调整)：
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1. **标题**: *Tenomodulin is required for tendon endurance running and collagen maturation*
**作者**: Docheva D, et al.
**摘要**: 研究通过基因敲除模型发现TNMD在肌腱胶原纤维成熟中起关键作用，重组TNMD蛋白可增强体外肌腱干细胞的胶原沉积能力，提示其在肌腱修复中的应用潜力。
2. **标题**: *Recombinant Tenomodulin promotes the osteogenic and tendinogenic differentiation of mesenchymal stem cells*
**作者**: Chen CH, et al.
**摘要**: 利用重组TNMD蛋白处理间充质干细胞，发现其通过激活BMP信号通路显著促进肌腱相关基因(如Scx、Col1a1)表达，并抑制成骨分化，表明TNMD在肌腱再生中的定向调控作用。
3. **标题**: *Efficient expression and purification of recombinant human Tenomodulin in CHO cells*
**作者**: Shukunami C, et al.
**摘要**: 开发了一种基于CHO细胞的重组TNMD蛋白高效表达与纯化系统，验证其生物活性，为大规模生产用于肌腱组织工程的TNMD蛋白提供技术方案。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“Tenomodulin recombinant protein”获取最新研究。
**Background of TNMD Recombinant Protein**
Tenomodulin (TNMD), encoded by the *TNMD* gene, is a type II transmembrane glycoprotein predominantly expressed in tendons and ligaments. It plays a critical role in tendon maturation, maintenance, and collagen fibril organization. TNMD is proteolytically cleaved to release its C-terminal domain, which exhibits anti-angiogenic properties by inhibiting endothelial cell migration and vessel formation, suggesting its involvement in regulating tissue vascularization during tendon development.
TNMD deficiency has been linked to tendon matrix abnormalities, reduced tenocyte proliferation, and accelerated aging, highlighting its importance in musculoskeletal health. Its expression is regulated by mechanical stress and transcription factors like Scleraxis, a key regulator of tendon differentiation.
Recombinant TNMD protein is engineered using expression systems (e.g., mammalian or bacterial cells) to produce purified, bioactive forms for research and therapeutic applications. It typically includes functional domains such as the conserved C-terminal cysteine-rich domain responsible for its anti-angiogenic and matrix-modulating activities. Studies utilize TNMD recombinant protein to explore tendon repair mechanisms, tissue engineering strategies, and its potential in treating tendon injuries or degenerative conditions like tendinopathy. Additionally, its role in modulating extracellular matrix components and interactions with growth factors (e.g., BMP-2) underscores its relevance in regenerative medicine.
Overall, TNMD recombinant protein serves as a vital tool for dissecting tendon biology and developing targeted therapies for musculoskeletal disorders.
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