| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT25 |
| Uniprot No | Q7Z3Z0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450aa |
| 氨基酸序列 | MSLRLSSASR RSCPRPTTGS LRLYGGGTSF GTGNSCGISG IGSGFSSAFG GSSSGGNTGG GNPCAGFTVN ERGLLSGNEK VTMQNLNDRL ASYLDSVHAL EEANADLEQK IKGWYEKFGP GSCRGLDHDY SRYFPIIDDL KNQIIASTTS NANAVLQIDN ARLTADDFRL KYENELALHQ SVEADVNGLR RVLDEITLCR TDLEIQYETL SEEMTYLKKN HKEEMQVLQC AAGGNVNVEM NAAPGVDLTV LLNNMRAEYE ALAEQNRRDA EAWFNEKSAS LQQQISEDVG ATTSARNELT EMKRTLQTLE IELQSLLATK HSLECSLTET ESNYCAQLAQ IQAQIGALEE QLHQVRTETE GQKLEYEQLL DIKLHLEKEI ETYCLLIGGD DGACKSGGYK SKDYGSGNVG SQVKDPAKAI VVKKVLEEVD QRSKILTTRL HSLEEKSQSN |
| 预测分子量 | 49,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT25重组蛋白的3篇参考文献的简要信息(注：文献为虚构示例，仅用于演示格式)：
1. **文献名称**：*Recombinant KRT25 Expression and Its Role in Hair Follicle Differentiation*
**作者**：Chen L, Wang X, et al.
**摘要**：研究利用昆虫细胞系统表达重组KRT25蛋白，证实其通过调控Wnt信号通路参与毛囊分化和角化过程。
2. **文献名称**：*Structural Characterization of Recombinant Human KRT25 and Its Pathogenic Mutations*
**作者**：Johnson R, Müller S.
**摘要**：通过大肠杆菌表达重组KRT25蛋白并进行晶体结构分析，揭示了与遗传性皮肤疾病相关的突变位点对蛋白稳定性的影响。
3. **文献名称**：*Recombinant KRT25 as a Biomarker for Esophageal Squamous Cell Carcinoma*
**作者**：Tanaka K, Li H, et al.
**摘要**：开发了基于哺乳动物细胞表达的重组KRT25蛋白，并发现其在食管鳞状细胞癌患者血清中异常高表达，提示其作为诊断标志物的潜力。
(注：以上文献为模拟内容，实际研究中请通过学术数据库检索具体文献。)
**Background of KRT25 Recombinant Protein**
KRT25 (Keratin 25) is a member of the keratin family, a group of structural proteins critical for maintaining the integrity of epithelial cells and appendages such as hair, nails, and skin. As a type I acidic keratin, KRT25 is primarily expressed in specialized epithelial tissues, including the hair follicle inner root sheath and the upper digestive tract. It plays a role in forming intermediate filaments, providing mechanical resilience and regulating cellular stress responses.
The recombinant KRT25 protein is engineered using biotechnological methods, such as expression in bacterial or mammalian systems, to produce a purified, functional form of the protein. This involves cloning the KRT25 gene into expression vectors, followed by protein purification techniques like affinity chromatography. Recombinant KRT25 retains the biological activity of its native counterpart, enabling researchers to study its interactions, structural properties, and role in diseases.
Interest in KRT25 stems from its association with epithelial disorders and hair-related pathologies. Mutations in keratin genes, including KRT25. are linked to tissue fragility syndromes, such as monilethrix (a hair shaft disorder), and may contribute to carcinogenesis in epithelial tissues. Recombinant KRT25 serves as a tool for investigating disease mechanisms, screening therapeutic compounds, and developing diagnostic biomarkers. Additionally, it aids in studying hair follicle biology, with potential applications in regenerative medicine and cosmetic research.
Ongoing research focuses on elucidating KRT25's post-translational modifications, interaction networks, and its utility in modeling epithelial diseases *in vitro*. Its recombinant form bridges foundational keratin biology with translational applications in dermatology and oncology.
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