| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSRP1 |
| Uniprot No | Q08945 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-709aa |
| 氨基酸序列 | MAETLEFNDVYQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGHVYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCTTGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSGLLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE |
| 预测分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SSRP1重组蛋白的3篇参考文献示例(注：文献为模拟生成，仅供参考方向)：
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1. **文献名称**：*Recombinant SSRP1 Expression and Its Role in Chromatin Remodeling*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组人源SSRP1蛋白，发现其在体外可增强组蛋白H2A-H2B二聚体的解离能力，证实了SSRP1在染色质动态重塑中的关键作用。
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2. **文献名称**：*Structural Analysis of SSRP1 Reveals DNA Binding Flexibility*
**作者**：Lee C, et al.
**摘要**：利用重组SSRP1蛋白进行X射线晶体学研究，解析了其HMG结构域与DNA结合的分子机制，发现SSRP1通过构象变化适应不同DNA结构，促进转录延伸复合体的稳定性。
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3. **文献名称**：*Functional Characterization of SSRP1 in the FACT Complex*
**作者**：Wang Y, et al.
**摘要**：通过共表达重组SSRP1和SUPT16H蛋白，重构了功能性FACT复合体，并证明SSRP1的C端结构域对维持复合体稳定性和核小体组装能力至关重要。
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**建议**：实际文献需通过PubMed、Web of Science等平台搜索关键词(如"SSRP1 recombinant" "FACT complex expression")，重点关注蛋白表达纯化、结构功能或机制研究类论文。
**Background of SSRP1 Recombinant Protein**
SSRP1 (Structure-Specific Recognition Protein 1) is a multifunctional chromatin-associated protein that plays a critical role in transcriptional regulation, DNA repair, and replication. It is a key component of the FACT (Facilitates Chromatin Transcription) complex, which also includes SUPT16H. The FACT complex is essential for maintaining chromatin integrity during processes like RNA polymerase II-mediated transcription, where it transiently disrupts histone-DNA interactions to enable transcriptional elongation without permanently dismantling nucleosomes.
SSRP1 contains an N-terminal dimerization domain, a middle domain involved in histone binding, and a C-terminal HMG (High Mobility Group) box domain that recognizes and binds to specific DNA structures, such as bent or distorted regions. This structural versatility allows SSRP1 to interact with both histones and DNA, facilitating chromatin remodeling. Recombinant SSRP1 protein is typically produced in vitro using expression systems like *E. coli* or mammalian cell lines, followed by purification steps (e.g., affinity chromatography) to ensure high purity and functionality.
Studies utilizing SSRP1 recombinant protein have illuminated its role in gene expression regulation, DNA damage response, and epigenetic modifications. Dysregulation of SSRP1 is linked to genomic instability and cancer progression, as aberrant FACT activity can promote oncogene transcription and chemoresistance. Additionally, SSRP1 interacts with transcription factors and co-activators, influencing stem cell pluripotency and differentiation.
Research applications of recombinant SSRP1 include in vitro chromatin remodeling assays, protein-protein interaction studies, and investigations into its role in replication stress. Its recombinant form enables precise biochemical characterization, aiding the development of targeted therapies for cancers reliant on FACT complex activity. Overall, SSRP1 recombinant protein serves as a vital tool for dissecting chromatin dynamics and developing epigenetic therapeutics.
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