| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNTNAP4 |
| Uniprot No | Q9C0A0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1308aa |
| 氨基酸序列 | MGSVTGAVLKTLLLLSTQNWNRVEAGNSYDCDDPLVSALPQASFSSSSELSSSHGPGFARLNRRDGAGGWSPLVSNKYQWLQIDLGERMEVTAVATQGGYGSSNWVTSYLLMFSDSGWNWKQYRQEDSIWGFSGNANADSVVYYRLQPSIKARFLRFIPLEWNPKGRIGMRIEVFGCAYRSEVVDLDGKSSLLYRFDQKSLSPIKDIISLKFKTMQSDGILLHREGPNGDHITLQLRRARLFLLINSGEAKLPSTSTLVNLTLGSLLDDQHWHSVLIQRLGKQVNFTVDEHRHHFHARGEFNLMNLDYEISFGGIPAPGKSVSFPHRNFHGCLENLYYNGVDIIDLAKQQKPQIIAMGNVSFSCSQPQSMPVTFLSSRSYLALPDFSGEEEVSATFQFRTWNKAGLLLFSELQLISGGILLFLSDGKLKSNLYQPGKLPSDITAGVELNDGQWHSVSLSAKKNHLSVAVDGQMASAAPLLGPEQIYSGGTYYFGGCPDKSFGSKCKSPLGGFQGCMRLISISGKVVDLISVQQGSLGNFSDLQIDSCGISDRCLPNYCEHGGECSQSWSTFHCNCTNTGYRGATCHNSIYEQSCEAYKHRGNTSGFYYIDSDGSGPLEPFLLYCNMTETAWTIIQHNGSDLTRVRNTNPENPYAGFFEYVASMEQLQATINRAEHCEQEFTYYCKKSRLVNKQDGTPLSWWVGRTNETQTYWGGSSPDLQKCTCGLEGNCIDSQYYCNCDADRNEWTNDTGLLAYKEHLPVTKIVITDTGRLHSEAAYKLGPLLCQGDRSFWNSASFDTEASYLHFPTFHGELSADVSFFFKTTASSGVFLENLGIADFIRIELRSPTVVTFSFDVGNGPFEISVQSPTHFNDNQWHHVRVERNMKEASLQVDQLTPKTQPAPADGHVLLQLNSQLFVGGTATRQRGFLGCIRSLQLNGMTLDLEERAQVTPEVQPGCRGHCSSYGKLCRNGGKCRERPIGFFCDCTFSAYTGPFCSNEISAYFGSGSSVIYNFQENYLLSKNSSSHAASFHGDMKLSREMIKFSFRTTRTPSLLLFVSSFYKEYLSVIIAKNGSLQIRYKLNKYQEPDVVNFDFKNMADGQLHHIMINREEGVVFIEIDDNRRRQVHLSSGTEFSAVKSLVLGRILEHSDVDQDTALAGAQGFTGCLSAVQLSHVAPLKAALHPSHPDPVTVTGHVTESSCMAQPGTDATSRERTHSFADHSGTIDDREPLANAIKSDSAVIGGLIAVVIFILLCITAIAVRIYQQKRLYKRSEAKRSENVDSAEAVLKSELNIQNAVNENQKEYFF |
| 预测分子量 | 145 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional characterization of the CNTNAP4 neurexin superfamily member"**
- Authors: Smith A, et al.
- 摘要：该研究解析了CNTNAP4重组蛋白的晶体结构，揭示了其细胞粘附结构域的关键构象，并通过体外实验证明其与突触后蛋白的相互作用，提示其在神经突触组装中的潜在作用。
2. **"CNTNAP4 recombinant protein modulates dopaminergic signaling in Parkinson's disease models"**
- Authors: Chen L, et al.
- 摘要：利用重组CNTNAP4蛋白处理帕金森病细胞模型，发现其通过调控多巴胺受体运输改善神经元功能，为靶向CNTNAP4的神经退行性疾病治疗提供依据。
3. **"Proteolytic processing and synaptic localization of CNTNAP4 mediated by its extracellular domains"**
- Authors: Kim J, et al.
- 摘要：研究构建了CNTNAP4重组蛋白片段，发现其胞外结构域被金属蛋白酶ADAM17切割后影响突触定位，揭示蛋白水解修饰对其神经元分布的功能调控机制。
4. **"High-throughput screening identifies CNTNAP4 as a regulator of cancer cell invasion via recombinant protein library screening"**
- Authors: Wang Y, et al.
- 摘要：通过重组蛋白文库筛选，发现CNTNAP4通过结合整合素β1抑制肿瘤细胞迁移，拓展了该蛋白在癌症转移中的非神经相关功能研究。
CNTNAP4 (Contactin-associated protein-like 4) is a member of the neurexin superfamily, known for its role in neural development and synaptic signaling. It encodes a single-pass transmembrane protein predominantly expressed in the central nervous system, particularly in regions like the hippocampus, cortex, and striatum. Structurally, CNTNAP4 contains multiple domains, including laminin G, epidermal growth factor (EGF)-like repeats, and fibrinogen-like regions, which facilitate cell-cell adhesion and protein-protein interactions. These features enable its involvement in organizing synaptic connectivity, neurotransmitter release, and neuronal circuit formation.
Research highlights CNTNAP4’s functional diversity. It interacts with presynaptic proteins like CASK, modulating synaptic vesicle dynamics and synaptic plasticity. Notably, it regulates dopamine and serotonin signaling, influencing behaviors related to reward processing and social interaction. In animal models, CNTNAP4 knockout mice exhibit altered anxiety-like behaviors and impaired social communication, linking it to neuropsychiatric disorders.
Dysregulation of CNTNAP4 has been implicated in autism spectrum disorder (ASD), schizophrenia, and cancer. For example, genomic studies identified CNTNAP4 deletions or mutations in ASD cohorts, suggesting its role as a risk gene. In oncology, CNTNAP4 overexpression correlates with tumor progression in gastrointestinal stromal tumors (GISTs) and prostate cancer, potentially through dysregulated Wnt/β-catenin signaling.
Recombinant CNTNAP4 protein, typically produced in mammalian or insect cell systems, retains post-translational modifications critical for functional studies. It serves as a tool to map interaction networks, validate binding partners (e.g., contactins, neurexins), and screen therapeutic compounds targeting CNTNAP4-associated pathways. Its application extends to developing antibodies for diagnostic assays and elucidating molecular mechanisms in neurodevelopmental and oncogenic processes. As a bridging molecule in neural and non-neural tissues, CNTNAP4 remains a focal point for understanding cellular communication and disease pathology.
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