| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SQLE |
| Uniprot No | Q14534 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-574aa |
| 氨基酸序列 | LSYRCRHRNGGLLGRQQSGSQFALFSDILSGLPFIGFFWAKSPPESENKEQLEARRRRKGTNISETSLIGTAACTSTSSQNDPEVIIVGAGVLGSALAAVLSRDGRKVTVIERDLKEPDRIVGEFLQPGGYHVLKDLGLGDTVEGLDAQVVNGYMIHDQESKSEVQIPYPLSENNQVQSGRAFHHGRFIMSLRKAAMAEPNAKFIEGVVLQLLEEDDVVMGVQYKDKETGDIKELHAPLTVVADGLFSKFRKSLVSNKVSVSSHFVGFLMKNAPQFKANHAELILANPSPVLIYQISSSETRVLVDIRGEMPRNLREYMVEKIYPQIPDHLKEPFLEATDNSHLRSMPASFLPPSSVKKRGVLLLGDAYNMRHPLTGGGMTVAFKDIKLWRKLLKGIPDLYDDAAIFEAKKSFYWARKTSHSFVVNILAQALYELFSATDDSLHQLRKACFLYFKLGGECVAGPVGLLSVLSPNPLVLIGHFFAVAIYAVYFCFKSEPWITKPRALLSSGAVLYKACSVIFPLIYSEMKYMVH |
| 预测分子量 | 63.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SQLE(角鲨烯环氧酶)重组蛋白的示例参考文献(注：以下为模拟文献，建议通过学术数据库查询真实研究)：
---
1. **文献名称**: *Heterologous Expression and Characterization of Recombinant Squalene Epoxidase in Escherichia coli*
**作者**: Zhang, L., et al.
**摘要**: 本研究成功在大肠杆菌中克隆并表达了SQLE重组蛋白，优化了表达条件并通过亲和层析纯化。酶活性分析表明重组SQLE具有催化角鲨烯转化为2.3-环氧角鲨烯的功能，为后续药物筛选提供了基础。
2. **文献名称**: *Structural Insights into Squalene Epoxidase through Cryo-EM Analysis*
**作者**: Tanaka, K., & Watanabe, T.
**摘要**: 通过冷冻电镜技术解析了SQLE重组蛋白的三维结构，揭示了其活性位点及底物结合区域的结构特征，为设计靶向SQLE的抑制剂(如抗真菌药物)提供了结构生物学依据。
3. **文献名称**: *Functional Role of SQLE in Cholesterol Biosynthesis: Implications for Hyperlipidemia*
**作者**: Gupta, R., et al.
**摘要**: 利用重组SQLE蛋白进行体外实验，证实其在胆固醇合成通路中的关键作用，并发现SQLE活性异常与高脂血症相关，提示其作为治疗靶点的潜力。
4. **文献名称**: *Development of a High-Throughput Assay for SQLE Inhibitors Using Recombinant Protein*
**作者**: Müller, S., et al.
**摘要**: 基于SQLE重组蛋白建立了高通量药物筛选平台，用于快速鉴定新型抗真菌化合物，验证了多个先导分子对SQLE酶活的抑制作用。
---
**建议**：如需真实文献，可通过PubMed、Web of Science等平台搜索关键词“squalene epoxidase recombinant”或“SQLE protein expression”获取最新研究。
**Background of SQLE Recombinant Protein**
Squalene epoxidase (SQLE), a key enzyme in the cholesterol biosynthesis pathway, catalyzes the conversion of squalene to 2.3-oxidosqualene, a critical step in sterol and triterpenoid biosynthesis. This endoplasmic reticulum membrane-associated enzyme plays a central role in lipid metabolism and cellular homeostasis. Dysregulation of SQLE has been implicated in various diseases, including hypercholesterolemia, cancers, and fungal infections, making it a potential therapeutic target. For instance, elevated SQLE expression is observed in certain tumors, where it supports rapid cell proliferation by maintaining membrane integrity and signaling molecule production.
Recombinant SQLE protein is produced using genetic engineering techniques, typically by expressing the *SQLE* gene in heterologous systems like *E. coli*, yeast, or mammalian cell lines. This allows large-scale production of purified, bioactive SQLE for functional studies, inhibitor screening, and structural analysis. Researchers utilize recombinant SQLE to investigate its enzymatic mechanisms, substrate interactions, and regulatory pathways. It also serves as a tool for developing cholesterol-lowering drugs (e.g., terbinafine analogs) or antifungal agents, as SQLE is essential in ergosterol synthesis in pathogenic fungi.
Recent advances in cryo-EM and X-ray crystallography have enabled detailed structural characterization of SQLE, revealing insights into its catalytic domain and membrane-binding regions. However, challenges remain in studying its full-length structure due to its hydrophobic nature and complex membrane association. Ongoing research focuses on designing isoform-specific inhibitors and understanding SQLE's role in drug resistance, particularly in cancer and fungal pathogens. The development of recombinant SQLE continues to drive innovations in both basic science and translational medicine.
×
