| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT7 |
| Uniprot No | P08729 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469aa |
| 氨基酸序列 | MSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVA VRSAYGGPVGAGIREVTINQSLLAPLRLDADPSLQRVRQEESEQIKTLNN KFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDIFEAQIAGLRG QLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINRRTAAENEFVVLKKD VDAAYMSKVELEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMD NSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDD LRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDA RAKQEELEAALQRAKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEE SRLAGDGVGAVNISVMNSTGGSSSGGGIGLTLGGTMGSNALSFSSSAGPG LLKAYSIRTASASRRSARD |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT7重组蛋白的模拟参考文献示例(注：文献为假设性示例，实际引用需查询真实数据库)：
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1. **文献名称**: *"Recombinant Expression and Purification of Human Keratin 7 for Epithelial Cancer Biomarker Studies"*
**作者**: Müller A, et al.
**摘要**: 该研究通过大肠杆菌系统表达重组人KRT7蛋白，优化纯化条件并获得高纯度蛋白，用于开发特异性抗体，验证其在多种上皮源性肿瘤组织中的表达差异。
2. **文献名称**: *"Functional Characterization of Recombinant KRT7 in Cell Motility Assays"*
**作者**: Chen L, et al.
**摘要**: 利用昆虫细胞表达系统制备重组KRT7.证实其过表达可增强肿瘤细胞的迁移能力，并揭示其通过调控细胞骨架蛋白参与转移的分子机制。
3. **文献名称**: *"Structural Analysis of Keratin 7 Using Recombinant Protein Crystallography"*
**作者**: Gupta S, et al.
**摘要**: 通过哺乳动物细胞表达重组KRT7并进行结晶，首次解析其关键结构域的三维构象，为设计靶向KRT7的小分子抑制剂提供结构基础。
4. **文献名称**: *"Development of a KRT7 Recombinant Protein-Based ELISA for Serological Diagnosis"*
**作者**: Wang Y, et al.
**摘要**: 构建基于重组KRT7的ELISA检测方法，评估其在胆管癌患者血清中的诊断价值，显示其敏感性和特异性优于传统标志物。
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**提示**：实际研究中建议通过PubMed、Web of Science等平台，以关键词“KRT7 recombinant protein”、“keratin 7 expression”检索最新文献。
Keratins are a diverse family of intermediate filament proteins essential for maintaining cellular structural integrity, mechanical stability, and stress resistance. KRT7. a type II keratin (cytokeratin 7), is primarily expressed in simple and glandular epithelial tissues. It forms heterodimers with type I keratins, such as KRT19. to assemble into filaments that regulate cell shape, organelle positioning, and signaling pathways. Dysregulation of KRT7 is closely associated with epithelial-derived cancers, where its overexpression serves as a diagnostic marker for adenocarcinomas (e.g., lung, breast, ovarian) and helps distinguish metastatic carcinomas.
Recombinant KRT7 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to produce purified, biologically active forms of the protein. Its production enables standardized studies of KRT7’s role in tumor progression, epithelial-mesenchymal transition, and drug resistance. Researchers utilize recombinant KRT7 to develop antibodies for immunohistochemistry, aiding in cancer subtyping and differential diagnosis. It also serves as a critical reagent in functional assays to explore keratin interactions, cellular stress responses, and metastasis mechanisms. However, achieving proper post-translational modifications (e.g., phosphorylation) may require mammalian expression systems, adding complexity to production. Despite challenges, recombinant KRT7 remains pivotal in advancing cancer diagnostics, biomarker discovery, and targeted therapies for epithelial malignancies.
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