| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT33A |
| Uniprot No | O76009 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-404aa |
| 氨基酸序列 | MSYSCGLPSL SCRTSCSSRP CVPPSCHGCT LPGACNIPAN VSNCNWFCEG SFNGSEKETM QFLNDRLASY LEKVRQLERD NAELENLIRE RSQQQEPLVC ASYQSYFKTI EELQQKILCS KSENARLVVQ IDNAKLASDD FRTKYETELS LRQLVESDIN GLRRILDELT LCRSDLEAQV ESLKEELLCL KQNHEQEVNT LRCQLGDRLN VEVDAAPTVD LNQVLNETRS QYEALVETNR REVEQWFATQ TEELNKQVVS SSEQLQSYQA EIIELRRTVN ALEIELQAQH NLRDSLENTL TESEARYSSQ LSQVQRLITN VESQLAEIRS DLERQNQEYQ VLLDVRARLE CEINTYRSLL ESEDCKLPSN PCATTNACDK STGPCISNPC GLRARCGPCN TFGY |
| 预测分子量 | 45,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为模拟示例，因KRT33A相关研究较为专业且可能较少，建议通过PubMed或Google Scholar等平台查询最新文献。
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1. **文献名称**：*Expression and Purification of Recombinant Human Keratin 33A in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：该研究描述了KRT33A基因的克隆与在大肠杆菌中的重组表达，优化了蛋白纯化条件，并验证了其结构稳定性，为后续功能研究提供材料基础。
2. **文献名称**：*Functional Characterization of KRT33A in Epithelial Cell Differentiation*
**作者**：Lee H, et al.
**摘要**：通过重组KRT33A蛋白的体外实验，揭示了其在表皮细胞分化中的作用机制，发现其与细胞骨架蛋白的相互作用对屏障功能至关重要。
3. **文献名称**：*Structural Analysis of Recombinant Keratin 33A Using Cryo-Electron Microscopy*
**作者**：Zhang R, et al.
**摘要**：利用冷冻电镜解析了重组KRT33A蛋白的三维结构，阐明了其与其他角蛋白异源二聚化的分子基础，为遗传性皮肤病研究提供结构依据。
4. **文献名称**：*KRT33A Recombinant Protein as a Potential Biomarker in Hair Follicle Disorders*
**作者**：Garcia M, et al.
**摘要**：评估了重组KRT33A在毛囊疾病中的表达变化，提出其作为诊断标志物的潜力，并通过体外模型验证了其在毛发再生中的功能。
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建议通过以下关键词扩展搜索：
`KRT33A recombinant expression`、`keratin 33A protein purification`、`KRT33A functional study`。
Keratin 33A (KRT33A) is a member of the keratin family, a group of structural proteins critical for maintaining the integrity of epithelial cells and forming intermediate filaments in the cytoskeleton. Specifically classified as a type I "hard" keratin, KRT33A is predominantly expressed in hair follicles and nails, where it contributes to the mechanical strength and resilience of these specialized tissues. Its gene, *KRT33A*, is located on human chromosome 17q21.2 within a cluster of other type I keratin genes. Dysregulation or mutations in keratins, including KRT33A, have been linked to hereditary skin fragility disorders, such as epidermolysis bullosa simplex, highlighting their essential role in cellular stress resistance.
Recombinant KRT33A protein is engineered using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to produce purified, functional keratin for research and therapeutic applications. This recombinant form enables studies on keratin filament assembly, protein-protein interactions, and mechanisms underlying keratin-related pathologies. It is particularly valuable for investigating hair follicle biology, as KRT33A mutations are associated with hair shaft abnormalities. Additionally, recombinant KRT33A serves as an antigen for antibody development, a substrate for drug screening, or a component in biomaterials aimed at tissue repair. Its production often involves codon optimization and affinity tagging (e.g., His-tag) to enhance yield and purification efficiency. By providing a controlled and scalable source of KRT33A, recombinant technology accelerates both basic research and translational efforts targeting keratin-associated diseases.
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